非小细胞肺癌患者外周血有核细胞miR-205-5p水平及其临床意义

发布时间：2018-03-06 12:38

本文选题：miR--p　切入点：外周血有核细胞　出处：《中国肿瘤生物治疗杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨非小细胞肺癌患者外周血有核细胞miR-205-5p的表达水平是否可作为分子标志物。方法:收集昆明医科大学第三附属医院2011年6月至2012年6月非小细胞肺癌首诊患者60例,其中无远处转移患者30例,有远处转移患者30例。另外收集健康志愿者30例作为对照组。qPCR技术检测人支气管上皮细胞BEAS-2B、云南个旧肺鳞癌细胞YTMLC-90、云南宣威肺腺癌细胞XWLC-05以及人外周血有核细胞的表达水平。分析外周血有核细胞miR-205-5p的表达水平与非小细胞肺癌患者血清标志物水平、TNM分期、淋巴结转移、远处转移等临床病理参数的相关性。结果:miR-205-5p在BEAS-2B细胞中表达水平低(0.820 8±0.255 3),而在YTMLC-90(17.507 2±1.906 3)和XWLC-05(5.725 2±1.012 0)细胞中表达水平明显升高,差异有统计学意义(P0.05);miR-205-p在健康人外周血中的表达水平显著低于无转移的以及转移的非小细胞肺癌患者(1.073 0 vs3.658 8、19.6324,均P0.01)。外周血有核细胞miR-205-5p的阳性表达率与非小细胞肺癌患者性别、年龄、淋巴结转移、病理类型和血清肿瘤标志物水平(CEA、CA125、CA242)无关(P0.05),而与患者远处转移、TNM分期、血清肿瘤标志物CA153水平明显相关(P0.01)。结论:miR-205-5p表达水平异常升高可能作为非小细胞肺癌患者转移相关的分子标志物。
[Abstract]:Objective: To investigate the expression level of patients with non-small cell lung cancer peripheral lymphocytes miR-205-5p whether can be used as molecular markers. Methods: from the Third Affiliated Hospital of Kunming Medical University from June 2011 to June 2012, 60 cases of non-small cell lung cancer patients first diagnosed, including 30 cases without distant metastasis patients, 30 cases of patients with metastasis in the distance. In addition to collect health 30 volunteers were used as control to detect human bronchial epithelial cells BEAS-2B group.QPCR technology, Yunnan Gejiu lung squamous carcinoma cells YTMLC-90, Yunnan Xuanwei lung adenocarcinoma XWLC-05 cells and human peripheral blood mononuclear cells. The expression level of the expression level of peripheral blood miR-205-5p cells in patients with non-small cell lung cancer serum markers. TNM staging, lymph node metastasis, distant metastasis and clinical pathological correlation parameters. Results: miR-205-5p expression in BEAS-2B cells of low level (0.8208 + 0.2553), and in YTML C-90 (17.5072 + 1.9063) and XWLC-05 (5.7252 + 1.0120) expression was significantly increased, the difference was statistically significant (P0.05); the expression of miR-205-p in human peripheral blood was significantly lower than that without metastasis and metastasis in patients with non-small cell lung cancer (vs3.658 1.0730 8,19.6324, P0.01) in peripheral blood. MiR-205-5p positive cells and the rate of non-small cell lung cancer patients with gender, age, lymph node metastasis, pathological type and the level of serum tumor markers (CEA, CA125, CA242) (P0.05), independent of transfer, and patients with distant TNM staging, serum tumor markers correlated significantly with CA153 level (P0.01) conclusion. Abnormal expression of miR-205-5p levels as possible in patients with non-small cell lung cancer metastasis related molecular markers.

【作者单位】：昆明医科大学第三附属医院/云南省肿瘤医院肿瘤研究所暨云南省肿瘤分子标志研究中心暨詹启敏院士工作站;
【基金】：国家自然科学基金资助项目(No.81560380,81460358,81060177) 云南省卫生科技项目资助(No.2014NS023,2014NS024) 詹启敏院士工作站基金资助(No.2015IC002)~~
【分类号】：R734.2

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