ERK5和MMP-9在骨肉瘤中的表达及其临床意义

发布时间：2018-03-06 15:00

本文选题：骨肉瘤　切入点：细胞外信号调节激酶　出处：《中国肿瘤临床》2017年14期 　论文类型：期刊论文

【摘要】：目的:探讨细胞外信号调节激酶5(extracellular signal-regulated kinase 5,ERK5)蛋白和基质金属蛋白酶-9(matrix metallo proteinase-9,MMP-9)在骨肉瘤组织中的表达及其临床意义。方法:选取71例骨肉瘤组织和40例正常骨组织,采用免疫组织化学法检测ERK5和MMP-9的表达,并分析ERK5和MMP-9的表达与骨肉瘤患者临床病理特征及预后之间的关系。结果:骨肉瘤组织中ERK5和MMP-9阳性表达率分别为85.9%(61/71)、74.65%(53/71),均明显高于正常骨组织中阳性表达率12.5%(5/40)、10.0%(4/40),差异均具有统计学意义(均P0.05)。ERK5和MMP-9的阳性表达与Enneking分期和转移相关(均P0.05)。Kaplan-Meier生存分析显示在骨肉瘤组织中ERK5、MMP-9的阳性表达组患者生存时间均短于阴性表达组(均P0.05)。单因素Cox回归分析发现,肿瘤大小、Enneking分期、转移、ERK5和MMP-9阳性表达与患者总生存时间相关(均P0.05);多因素分析证实Enneking分期、转移、ERK5和MMP-9蛋白的阳性表达可作为骨肉瘤患者独立的预后因子(均P0.05)。结论:ERK5和MMP-9在骨肉瘤组织中高表达,与骨肉瘤的病理特征及预后相关;二者可能在骨肉瘤发生发展的过程中起着重要作用。
[Abstract]:Objective: To investigate the expression of extracellular signal regulated kinase 5 (extracellular signal-regulated 5 kinase, ERK5) protein and matrix metalloproteinase -9 (matrix metallo proteinase-9, MMP-9) expression in osteosarcoma and its clinical significance. Methods: 71 cases of osteosarcoma and 40 cases of normal bone tissue, the expression of ERK5 and MMP-9 were detected by immunohistochemistry the chemical method, and analyze the relationship between ERK5 and MMP-9 expression in patients with osteosarcoma and the clinical pathological features and prognosis of osteosarcoma. Results: ERK5 and MMP-9 positive expression rate was 85.9% (61/71), 74.65% (53/71), were significantly higher than that of normal bone tissue positive expression rate of 12.5% (5/40), 10% (4/40), the differences were statistically significant (P0.05) positive expression of Enneking and.ERK5 and MMP-9 staging and metastasis (P0.05).Kaplan-Meier survival analysis showed that ERK5 in osteosarcoma, MMP-9 positive The expression of patients survival time was shorter than the negative expression group (P0.05). Single factor Cox regression analysis showed that tumor size, Enneking stage, metastasis, ERK5 and MMP-9 positive expression and overall survival time (P0.05); multivariate analysis showed that Enneking staging, metastasis, the positive expression of ERK5 and MMP-9 protein can be as the independent prognostic factor in patients with osteosarcoma (P0.05). Conclusion: ERK5 and MMP-9 expression in osteosarcoma tissues, and pathological characteristics and prognosis of osteosarcoma; plays an important role in the development process of the two may occur in osteosarcoma.

【作者单位】：兰州大学第二医院骨科;甘肃省骨关节疾病研究重点实验室;甘肃省肿瘤医院骨软组织肿瘤科;
【基金】：国家自然科学基金(编号:81672207)资助~~
【分类号】：R738.1

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