HHLA2和PD-L1在非小细胞肺癌组织中的表达及临床意义

发布时间：2018-03-08 16:30

本文选题：人内源性逆转录病毒-H长末端重复关联蛋白2　切入点：程序性死亡配体1　出处：《西南医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:肿瘤微环境中的重要免疫分子人内源性逆转录病毒-H长末端重复关联蛋白2(human endogenous retrovirus subfamily H long terminal repeat associating protein 2,HHLA2)和程序性死亡配体1(programmed celldeath-ligand 1,PD-L1),在肿瘤的发生、发展过程中发挥重要的调控作用。本研究通过检测非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中HHLA2和PD-L1的表达情况,并分析其与非小细胞肺癌患者临床病理特征间的关系,探讨HHLA2和PD-L1在非小细胞肺癌的发生、发展、浸润及转移过程中的作用。方法:选取西南医科大学附属第一医院病理科2015年1月至2015年12月手术切除的60例(肺腺癌30例,肺鳞癌30例)NSCLC石蜡包埋存档组织块,据手术切除标本大小,严格按照距NSCLC肿瘤组织灶3cm的标准取癌旁肺组织,共30例(鳞癌18例、腺癌12例)作为癌旁肺组织对照组,所有入选病例均排除其他肺部疾病及其他组织、器官的恶性肿瘤,术前未行化疗、放疗、免疫治疗及其他针对恶性肿瘤的治疗;并选取30例经病理确诊的正常肺组织为对照组。采用免疫组织化学En Vision法检测NSCLC组织中HHLA2与PD-L1的表达情况,并比较非小细胞肺癌的HHLA2和PD-L1表达在年龄、病理分型、分期、吸烟情况和性别间的差异。结果:1.NSCLC组织中HHLA2的阳性表达率为81.67%,明显高于其在癌旁肺组织上皮细胞中的阳性表达率13.33%和正常肺组织上皮细胞中的阳性表达率0.00%,且差异有统计学意义(P0.05);而HHLA2在正常肺组织上皮细胞及癌旁肺组织上皮细胞中的表达差异无统计学意义(P0.05),HHLA2表达与NSCLC的临床分期、淋巴结转移、性别、年龄、吸烟情况及病理分型无相关性。2.NSCLC组织中PD-L1的阳性表达率为53.33%,明显高于其在癌旁肺组织上皮细胞中的阳性表达率(0.00%)和正常肺组织上皮细胞中的阳性表达率(0.00%),且差异有统计学意义(P0.05);PD-L1表达与NSCLC的临床分期(P0.05)、淋巴结转移(P0.05)相关,但与其性别、年龄、吸烟情况、病理分型无相关性。3.NSCLC组织中HHLA2和PD-L1的表达具有相关性(r=0.503,P0.01)。结论:1.非小细胞肺癌组织中HHLA2的表达显著增高,可能成为免疫检查及治疗的新靶点。2.非小细胞肺癌组织中PD-L1的表达显著增高,与TNM分期有关,提示在NSCLC的发生、发展、浸润及转移过程中发挥了重要作用。3.HHLA2及PD-L1在非小细胞肺癌组织中的表达呈正相关,提示它们可能在NSCLC的发生、发展过程中发挥协同作用。
[Abstract]:Objective: an important immune molecule of human endogenous retrovirus long terminal repeat associated protein -H in tumor microenvironment (2 human endogenous retrovirus subfamily H long terminal repeat associating protein 2, HHLA2) and programmed death ligand 1 (programmed celldeath-ligand 1, PD-L1), in the incidence of cancer, play an important role in the process of development. This study through the detection of non-small cell lung cancer (non-small cell lung cancer, NSCLC) expression of HHLA2 and PD-L1 in tissues, and analyze its relationship with clinical pathological features of patients with non-small cell lung cancer. The study of HHLA2 and PD-L1 in non-small cell lung cancer occurrence, development, invasion and metastasis process. The first Affiliated Hospital from Southwestern Medical School pathology from January 2015 to December 2015 for 60 cases surgery (30 cases, 30 cases of lung adenocarcinoma and lung squamous cell carcinoma) NSCLC paraffin embedding Tissue, according to surgical specimen size, the adjacent lung tissue from the tumor foci in strict accordance with the NSCLC 3cm standard, a total of 30 cases (18 cases of squamous cell carcinoma, 12 cases of adenocarcinoma of lung tissue adjacent to cancer) as the control group, all patients were excluded other diseases of the lungs and other organs of the organization, not malignant tumor. Preoperative radiotherapy, chemotherapy, immunotherapy and the other for the treatment of malignant tumor; and selected 30 cases of normal lung tissue by pathology were as control group. The expression of HHLA2 and PD-L1 in NSCLC tissues was detected by immunohistochemical En Vision method, and compare the expression of HHLA2 in non small cell lung cancer and PD-L1 in age. Pathological type, stage, smoking status and gender differences. Results: the positive expression of HHLA2 in 1.NSCLC tissues was 81.67%, significantly higher than the positive expression in adjacent lung epithelial cells in rate of 13.33% and the normal lung tissues in epithelial cells The positive expression rate of 0%, and the difference was statistically significant (P0.05); while there was no significant difference in the expression of HHLA2 in epithelial cancer cells and normal lung tissue adjacent lung tissue in epithelial cells (P0.05), the expression of HHLA2 NSCLC and clinical staging, lymph node metastasis, gender, age, smoking status and expression of PD-L1 pathology there was no significant correlation between.2.NSCLC tissues was 53.33%, significantly higher than the positive expression in adjacent lung epithelial cells in the positive expression rate (0%) and normal lung epithelial cells in the rate (0%), and the difference was statistically significant (P0.05); the expression of PD-L1 with clinical stage NSCLC (P0.05). Lymph node metastasis (P0.05), but their gender, age, smoking status, with a correlation between the expression of HHLA2 and PD-L1 had no correlation with pathological type in.3.NSCLC tissues (r=0.503, P0.01). Conclusion: 1. the expression of HHLA2 in non small cell lung cancer was significantly increased High, it may become a new target for immune examination and treatment of the.2. expression of PD-L1 in non-small cell lung cancer significantly associated with TNM stage, suggesting that the occurrence of NSCLC in the development, invasion and metastasis, plays an important role in.3.HHLA2 and PD-L1 expression in non-small cell lung cancer tissues, suggesting that they in NSCLC, play a synergistic role in the process of development.

【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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