维吾尔族口腔鳞癌患者中PTEN和CK19的表达及其临床意义

发布时间：2018-03-10 03:21

本文选题：口腔鳞癌　切入点：PTEN　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:口腔鳞状细胞癌(Oral Squamous Cell Carcinoma,OSCC)是全球10大恶性肿瘤之一,它不仅危及患者的生命,还影响其美观和生存质量。虽然手术联合放化疗技术在不断完善,晚期OSCC患者的5年生存率只有40%~50%,但是早期的5年生存率高达85.4%。因此,OSCC的早期诊断对提高其疗效至关重要。多种肿瘤标记物(Tumor Markers,TM)联合检测对于恶性肿瘤的早期诊断和监测非常有价值。本研究分析维吾尔族口腔鳞状细胞癌患者中抑癌基因PTEN和细胞角蛋白19(CK19)的表达情况,探讨其在口腔鳞癌发生、发展中的作用及临床意义。方法:采用免疫组化EnVision法对病理确诊的102例OSCC及99例口腔良性肿瘤石蜡标本中PTEN、CK19蛋白进行检测,并采用SPSS22.0软件包对数据进行统计学处理,分析蛋白表达与临床病理特征的关系。结果:PTEN在OSCC组和对照组中阳性率分别为37.26%(38/102)和98.99%(98/99),PTEN在OSCC组织中表达显著降低或缺失(P0.001),但PTEN与肿瘤病理、临床分期相关性无统计学意义。CK19在鳞癌组表达阳性率为43.14%(44/102),其表达与肿瘤分化程度、恶性程度及临床分期存在相关性(P0.005),OSCC分化程度越低,CK19表达越高。在良性对照的不同组织之间CK19表达不同。结论:PTEN和CK19参与了OSCC的发生、发展、转移过程,PTEN缺失和CK19表达增强可能是OSCC预后不良的指征;两者可作为OSCC的补充肿肿瘤标记物,其联合检测在临床中具有应用价值。
[Abstract]:Objective: oral Squamous Cell carcinoma (OSCC) is one of the top 10 malignant tumors in the world. It not only endangers the life of patients, but also affects their beauty and quality of life. The 5-year survival rate of advanced OSCC patients is only 40%, but the early 5-year survival rate is as high as 85.4%. Therefore, the early diagnosis of OSCC is very important to improve its efficacy. The combined detection of multiple tumor markers (Tumor Markers TMN) is of great importance in the early diagnosis of malignant tumors. This study analyzed the expression of tumor suppressor gene PTEN and cytokeratin 19 (CK19) in Uygur oral squamous cell carcinoma (OSCC). To investigate the role and clinical significance of PTENCK19 protein in carcinogenesis and development of oral squamous cell carcinoma (OSCC) methods: immunohistochemical EnVision method was used to detect PTENNCK19 protein in 102 cases of OSCC and 99 cases of paraffin wax specimens of oral benign tumors. The data were processed by SPSS22.0 software package. Results the positive rates of OSCC group and control group were 37.26 / 102 and 98.9999 / 99 / 99 respectively. The expression of PTEN was significantly decreased or missing in OSCC tissues. However, PTEN was associated with tumor pathology. The positive rate of CK19 expression in squamous cell carcinoma group was 43.14% or 44 / 102%, and its expression was associated with the degree of tumor differentiation. There was a correlation between malignant degree and clinical stage. The lower the differentiation degree of P0.005 OSCC, the higher the expression of CK19. The expression of CK19 was different in different tissues of benign control. Conclusion: PTEN and CK19 are involved in the occurrence and development of OSCC. PTEN deletion and increased expression of CK19 may be the indications of poor prognosis of OSCC during metastasis, and they can be used as complementary tumor markers of OSCC, and their combined detection is valuable in clinical practice.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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