WIF1在膀胱癌中的表达及其与MMP-9的相关性分析

发布时间：2018-03-11 15:14

本文选题：膀胱癌　切入点：wnt信号　出处：《内蒙古大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测WIF1在膀胱癌组织与血清中的表达水平,并分析其与MMP-9的相关性,探讨其在膀胱癌的发生发展中的调节机制。方法:1、收集病例,分为实验组与对照组,实验组为膀胱癌组织,对照组为膀胱癌旁正常组织;实验组被分为高分化组与低分化组及浸润组与非浸润组。2、Real-time PCR检测WIF1在膀胱癌组织与癌旁正常组织、高分化组与低分化组、浸润组与非浸润组的基因表达。4、ELISA检测WIF1在膀胱癌高分化组与低分化组、浸润组与非浸润组血清蛋白表达。5、分别检测WIF1与MMP-9在膀胱癌浸润组的血清蛋白含量,分析二者的相关性。结果:1、在实验组与癌旁对照组检测WIF1基因表达水平显示,实验组的基因表达量(0.23 ±0.131)明显低于癌旁对照组(0.54±0.170),差异具有统计学意义(t=-0.710、P0.001);在低分化组WIF1基因表达量(0.16 ± 0.082)明显低于高分化组(0.26±0.088),差异具有统计学意义(t=-3.135、P0.01);在浸润组WIF1基因表达量(0.14±0.087)明显低于非浸润组(0.28±0.135),差异具有统计学意义(t=-3.204、P0.01)。2、WIF1在血清中蛋白表达检测显示,在低分化组的表达水平(21.85±5.833)明显低于高分化组(30.35±8.040),差异具有统计学意义(t=-3.203、P0.01);WIF1蛋白在浸润组的表达量(21.56±5.141)明显低于非浸润组(31.97土9.064),差异具有统计学意义(t=-3.599、P0.01);在浸润组,WIF1蛋白的表达与MMP-9蛋白表达具有相关性,呈负相关(r=-0.643、p0.05)。结论:1.WIF1与膀胱癌的发生、侵袭浸润密切相关,其在膀胱癌的发生、侵袭浸润中可能发挥重要调节功能,可能是通过调节经典wnt信号通路发挥作用。2.WIF1在血清中的异常表达可能为临床的早期发现提供有效证据,可能为膀胱癌的病理分级、侵袭浸润能力及术后预后的判断提供简单有效的检测方法。3.在膀胱癌的侵袭浸润中,WIF1可能通过促进MMP-9的表达进而发挥其侵袭浸润功能,其可能通过激活经典wnt信号通路发挥作用,或二者之间存在独立信号机制。
[Abstract]:Objective: to detect the expression of WIF1 in bladder cancer tissues and serum, and to analyze its correlation with MMP-9, and to explore its regulatory mechanism in the occurrence and development of bladder cancer. Methods: one case was collected and divided into experimental group and control group. The experimental group was divided into high differentiation group and low differentiation group, and infiltration group and non-invasive group. Real-time PCR was used to detect WIF1 in bladder cancer tissue and adjacent normal tissue, high differentiation group and low differentiation group. The expression of WIF1 was detected by Elisa in high differentiation and low differentiation groups, infiltration group and non invasive group. The serum protein levels of WIF1 and MMP-9 in bladder cancer infiltrating group were detected respectively. Results: the expression level of WIF1 gene was detected in the experimental group and the adjacent control group. The expression of WIF1 gene in the experimental group (0.23 卤0.131) was significantly lower than that in the control group (0.54 卤0.170), the difference was statistically significant, and the expression of WIF1 gene in the poorly differentiated group (0.16 卤0.082) was significantly lower than that in the well-differentiated group (0.26 卤0.088). The expression level of WIF1 in serum was significantly lower than that in non-invasive group (0.14 卤0.087), and the difference was statistically significant. The expression level of WIF1 protein in the poorly differentiated group (21.85 卤5.833) was significantly lower than that in the well-differentiated group (30.35 卤8.040), the difference was statistically significant (P < 21.56 卤5.141), and the expression of WIF1 protein in the infiltrating group was significantly lower than that in the non-invasive group (31.97 卤9.064), the difference was statistically significant (P 0.01), and the expression of WIF1 protein in the infiltrating group was significantly lower than that in the non-invasive group. The expression of MMP-9 was correlated with the expression of MMP-9 protein. Conclusion: 1. WIF1 is closely related to the occurrence and invasion of bladder cancer, which may play an important regulatory role in the occurrence and invasion of bladder cancer. The abnormal expression of WIF1 in serum may provide effective evidence for early clinical discovery and may be the pathological grade of bladder cancer. Evaluation of invasive invasion ability and postoperative prognosis provides a simple and effective method for detecting invasion and invasion of bladder cancer. WIF1 may play an invasive and invasive function by promoting the expression of MMP-9 in bladder cancer invasion and invasion. It may play a role by activating the classical wnt signaling pathway, or there is an independent signal mechanism between the two.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.14

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