Notch3、DLL1、CD133在结直肠腺癌组织中的表达及意义

发布时间：2018-03-12 16:39

本文选题：结直肠腺癌　切入点：Notch3　出处：《遵义医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测Notch3、DLL1、CD133在正常人结直肠组织、结直肠腺瘤、结直肠腺癌组织中的表达情况,探讨以上指标在结直肠癌发生、发展过程中的作用及其与临床病理特征间的关系。方法:采用免疫组织化学的方法检测12例正常结直肠黏膜、30例结直肠腺瘤、50例结直肠腺癌组织中Notch3、DLL1、CD133的表达情况,分析上述指标的表达与临床病理特征间的关系,并运用Spearman相关分析法对3组指标的表达情况进行相关性分析。结果:在正常结直肠黏膜、结直肠腺瘤、结直肠腺癌组织中Notch3的阳性表达率分别为8.3%(1/12)、26.7%(8/30)、64%(32/50);DLL1的阳性表达率分别为16.7%(2/12)、33.3%(10/30)、68.0%(34/50);CD133的阳性阳性表达率分别为8.3%(1/12)、36.7%(11/30)、54.0%(27/50)。上述指标在腺癌组的阳性表达率均明显高于腺瘤组及正常组,差异具有统计学意义(P0.05);腺瘤组与正常组相比,差异均无统计学意义(P0.05)。Notch3、DLL1、CD133在结直肠腺癌组织中的表达与性别、年龄、肿瘤部位、肿瘤大小及分化程度无关(P0.05),而均与淋巴结转移有关,伴有淋巴结转移组阳性表达率明显高于无淋巴结转移组(P0.05),且Notch3和DLL1的表达与Dukes分期有关,Dukes分期分期越高阳性表达率越高(P0.05),此外DLL1的表达与肿瘤浸润深度也有关,肿瘤浸润深度越深阳性表达率越高(P0.05)。Notch3蛋白和CD133蛋白表达呈正相关关系(r=0.478,P0.01)。其余指标间均无明显相关性。结论:1.Notch3、DLL1、CD133在正常结直肠组织、结直肠腺瘤、结直肠腺癌组织中的表达呈逐渐递增趋势,他们可能参与了结直肠癌的发生、发展过程。2.Notch3蛋白表达与淋巴结转移和Dukes分期有关,DLL1蛋白表达与肿瘤浸润深度和淋巴结转移有关,CD133蛋白表达与淋巴结转移有关;提示以上3个指标可有望成为新的肿瘤标志物推测结直肠癌患者的预后。3.Notch3蛋白与CD133蛋白的表达存在明显正相关关系。
[Abstract]:Objective: to detect the expression of Notch3DLL1CD133 in normal human colorectal tissues, colorectal adenomas and colorectal adenomas, and to investigate the occurrence of these markers in colorectal cancer. Methods: immunohistochemical method was used to detect the expression of Notch3- DL1 CD133 in 30 cases of colorectal adenoma and 50 cases of colorectal adenocarcinoma. To analyze the relationship between the expression of the above indexes and the clinicopathological features, and to analyze the correlation between the expression of the above indexes and the clinicopathological characteristics of the three groups by Spearman correlation analysis. Results: in normal colorectal mucosa, colorectal adenoma, The positive expression rates of Notch3 in colorectal adenocarcinoma tissues were 8.31 / 12 / 26.7 and 8 / 30 / 32 / 30, respectively. The positive expression rates of DLL1 in colorectal adenocarcinoma tissues were 16.775 / 12 / 33. 3 / 10 / 30 / 68.0 / 34 / 34 / 50 CD133, respectively. The positive expression rates of the above indexes in adenoma group were significantly higher than those in adenoma group and normal group, and the positive expression rate was 8.31 / 12 / 36.7T / 11 / 3054.30 / 27500.The positive expression rate of these indexes in adenocarcinoma group was significantly higher than that in adenoma group and normal group, and the positive expression rate of DLL1 in adenocarcinoma group was significantly higher than that in adenoma group and normal group, and the positive expression rate of DLL1 in adenocarcinoma group was significantly higher than that in adenoma group and normal group. There was no significant difference between adenoma group and normal group. The expression of CD133 in colorectal adenocarcinoma was not related to sex, age, tumor location, tumor size and differentiation degree, but was related to lymph node metastasis. The positive expression rate of Notch3 and DLL1 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis, and the expression of Notch3 and DLL1 was related to the Dukes staging. The higher the positive rate of Dukes staging was, the higher the positive expression rate of DLL1 was. In addition, the expression of DLL1 was also related to the depth of tumor invasion. The deeper the depth of tumor invasion, the higher the positive expression rate of P0.05N. Notch3 protein and CD133 protein expression. There was no significant correlation between the other markers. Conclusion: 1. Notch3 DLL1 CD133 in normal colorectal tissue and colorectal adenoma. The expression of colorectal adenocarcinoma is increasing gradually, and they may be involved in the development of colorectal cancer. 2. The expression of Notch3 protein was related to lymph node metastasis and Dukes stage. The expression of DLL1 protein was related to the depth of tumor invasion and lymph node metastasis, and the expression of CD133 protein was related to lymph node metastasis. The results suggest that the above three markers may be a new tumor marker for predicting a positive correlation between the expression of CD133 protein and the prognosis of colorectal cancer patients. 3.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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