人中心体蛋白Cep57的生物信息学分析

发布时间：2018-03-13 08:04

本文选题：人　切入点：中心体　出处：《生物技术》2017年06期 　论文类型：期刊论文

【摘要】：[目的]对人中心体蛋白Cep57(Centrosomal Protein 57)的理化性质、亲疏水性、跨膜区域、信号肽区域、二级结构、三级结构、蛋白质之间的相互作用、亚细胞定位进行预测分析。[方法]采用生物信息学方法,使用多种分析软件对Cep57进行预测分析。[结果]Cep57由500个氨基酸组成,等电点为9.35,在哺乳动物中较为保守;二级结构预测发现9个α螺旋和3个β折叠片层,三级结构预测结果的可靠性为100%,拉曼图分析表明预测结构稳定;亚细胞定位主要分布于细胞质及细胞核。[结论]人中心体蛋白Cep57是一个存在2种核定位序列的不稳定亲水蛋白,在细胞内分布范围广泛,参与维持细胞骨架的稳定及细胞周期的调控等生理活动。
[Abstract]:[objective] to study the physical and chemical properties, hydrophobicity, transmembrane region, signal peptide region, secondary structure, tertiary structure and protein interaction of human centrosomal protein (Cep57(Centrosomal Protein 57). [methods] Cep57 was predicted and analyzed by bioinformatics and multiple analysis softwares. [results] Cep57 was composed of 500 amino acids and its isoelectric point was 9.35, which was conserved in mammals. 9 伪 helix and 3 尾 folded lamellar layers were found in the secondary structure prediction. The reliability of the prediction results of the tertiary structure was 100. The Raman analysis showed that the predicted structure was stable. [conclusion] Human centrosomal protein (Cep57) is an unstable hydrophilic protein with two nuclear localization sequences. Participate in the maintenance of cytoskeleton stability and cell cycle regulation and other physiological activities.
【作者单位】：天津医科大学肿瘤医院国家肿瘤临床医学研究中心天津市肿瘤防治重点实验室天津市恶性肿瘤临床医学研究中心;
【基金】：国家自然科学基金项目(“EHD2介导53BP1 DNA损伤修复及其在乳腺癌发生发展中的作用机制研究”,No.81301711)
【分类号】：R730.2

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