端粒保护蛋白POT1在非小细胞肺癌组织中表达的研究

发布时间：2018-03-13 23:03

本文选题：非小细胞肺癌　切入点：端粒保护蛋白　出处：《中国人民解放军医学院》2016年博士论文　论文类型：学位论文

【摘要】：目的：研究端粒保护蛋白POT1 (protection of telomeres 1) mRNA在非小细胞肺癌中的表达情况。分析端粒保护蛋白POT1在非小细胞肺癌中的表达的改变与非小细胞肺癌的病理分型、分级以及临床之间的关系。方法：收集60例非小细胞肺癌组织标本及癌旁组织标本。实验分为两组：非小细胞肺癌组织组和癌旁组织组。提取各组标本的总RNA,通过半定量逆转录-聚合酶链反应法(reverse transcription polymerase chain reaction, RT-PCR)对POT1 mRNA在不同组织中的表达情况进行检测。结果：端粒保护蛋白POT1 mRNA在非小细胞肺癌组织组中的光密度比值为0.16114-0.0469,在癌旁组织组中的光密度比值为0.2632±0.1061,端粒保护蛋白POT1 mRNA在非小细胞肺癌组织组中的光密度比值明显低于癌旁组织,组间差异具有统计学意义(P0.05)。端粒保护蛋白POT1 mRNA的表达在性别、年龄、是否长期吸烟、分化程度、淋巴结是否转移、病理TNM分期EGFR基因突变、KRAS基因突变等的对比中均无显著差异(P0.05)。结论：端粒保护蛋白POT1 mRNA在非小细胞肺癌组织组中的相对表达量低于其在癌旁组织组中的表达量.端粒保护蛋白POT1与非小细胞肺癌患者性别、年龄、是否长期吸烟、分化程度、淋巴结是否转移、病理TNM分期、EGFR基因突变、KRAS基因突等无相关性。
[Abstract]:Objective: to study the expression of POT1 protection of telomeres 1 mRNA in non-small cell lung cancer (NSCLC). Methods: 60 specimens of non-small cell lung cancer (NSCLC) and paracancerous tissues (NSCLC) were collected and divided into two groups: NSCLC tissue group and paracancerous tissue group. The expression of POT1 mRNA in different tissues was detected by reverse transcription polymerase chain reaction.Results: the optical density ratio of telomere protection protein POT1 mRNA in non-small cell lung cancer tissues was 0.16114-0.0469. The ratio of optical density in paracancerous tissues was 0.2632 卤0.1061, and the ratio of telomeric protective protein POT1 mRNA in non-small cell lung cancer tissues was significantly lower than that in paracancerous tissues. The expression of telomere protection protein POT1 mRNA was found in sex, age, long-term smoking, differentiation, lymph node metastasis. There was no significant difference between EGFR gene mutation and KRAS gene mutation in pathological TNM staging. Conclusion: the relative expression of POT1 mRNA in non-small cell lung cancer is lower than that in paracancerous tissue. Telomere Protective protein POT1 and Sex in patients with Non-Small Cell Lung Cancer, There was no correlation among age, long-term smoking, differentiation, lymph node metastasis and pathological TNM staging.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R734.2

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