外周血游离DNA含量和完整性的动态变化检测在胃癌诊治中的意义

发布时间：2018-03-14 14:29

本文选题：游离DNA　切入点：DNA完整性　出处：《肿瘤防治研究》2017年02期 　论文类型：期刊论文

【摘要】：目的监测胃癌患者外周血游离DNA(cell-free DNA,cf-DNA)的含量,探讨其在胃癌诊治和预后中的价值。方法收集81例原发胃癌、18例胃良性肿瘤、19例胃癌前病变、43例同期门诊健康体检者以及部分术后和随访的外周血标本,提取游离DNA,荧光定量PCR检测其含量(ALU115和ALU247)并计算完整性(ALU247/ALU115),分析血清游离DNA和血浆游离DNA含量之间的相关性。结果血浆游离DNA含量和完整性在四组之间的差异有统计学意义(ALU115、ALU247以及完整性的P值均0.001),术前术后的差异也有统计学意义(P值分别为0.000、0.013、0.010),随访前后完整性差异有统计学意义(P=0.029),在有无转移、分期、分化程度间差异有统计学意义(P值分别为0.013、0.042、0.003)。血浆和血清游离DNA含量具有相关性且血浆离散趋势更小。ALU247的ROC曲线下面积最高(0.985)。结论游离DNA检测在胃癌的诊治和预后中有一定的价值。
[Abstract]:The purpose of monitoring patients with gastric cancer peripheral blood free DNA (cell-free DNA, cf-DNA) content, and explore the diagnosis and treatment and prognosis of gastric carcinoma. Methods 81 cases of primary gastric carcinoma, 18 cases of gastric benign tumors, 19 cases of precancerous lesions, 43 cases of healthy year outpatient service and part of the postoperative and follow-up peripheral blood samples, extract free DNA, fluorescence quantitative PCR to detect the content of (ALU115 and ALU247) and integrity (ALU247/ALU115), analyze the correlation between the content of free DNA in serum free DNA and plasma. Results there were significant differences in plasma free DNA content and integrity between the four groups (ALU115, ALU247 and integrity the P value was 0.001), preoperative postoperative difference was statistically significant (P = 0.000,0.013,0.010), statistically significant difference before and after complete follow-up (P=0.029), in metastasis, staging, there was statistically significant difference between the degree of differentiation Meaning (P = 0.013,0.042,0.003). The free DNA content in plasma and serum is correlated and the plasma dispersion trend is smaller. The area under the ROC curve of.ALU247 is the highest (0.985). Conclusion free DNA detection has certain value in the diagnosis and prognosis of gastric cancer.

【作者单位】：苏州大学附属第一医院临床检测中心;
【基金】：2015年度苏州市科技发展计划(应用基础研究-医疗卫生)(SYSD2015078)
【分类号】：R735.2

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