不同分子分型乳腺癌组织中趋化因子CXCL12及其受体CXCR4和CXCR7的表达及临床意义

发布时间：2018-03-14 19:34

本文选题：乳腺肿瘤　切入点：分子分型　出处：《中南大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨趋化因子CXCL12及其受体CXCR4和CXCR7在不同分子分型乳腺癌组织中的表达及临床意义。方法:应用实时定量PCR检查80例不同分子分型乳腺癌组织中的趋化因子CXCL12 mRNA及其受体CXCR4和CXCR7 mRNA的表达,采用免疫组织化学技术检测160例不同分子分型乳腺癌细胞组织中趋化因子CXCL12蛋白及其受体CXCR4和CXCR7蛋白的表达,并分析它们在不同分子分型乳腺癌中的表达差异。结果:CXCL12 mRNA及其受体CXCR4和CXCR7 mRNA在Luminal A和Luminal B型中表达无差异,在HER-2过表达型和三阴性乳腺癌(triple negative breast cancer,TNBC)两个类型之间的表达也无差异,但三者在HER-2过表达型和TNBC中的表达明显高于Luminal A和Luminal B型(均P0.05);CXCL12蛋白、CXCR4蛋白和CXCR7蛋白在HER-2过表达型和TNBC中的阳性表达率均明显高于Luminal A和Luminal B型(均P0.05),但在Luminal A和Luminal B之间及HER-2过表达型和TNBC之间的表达无差异。结论:趋化因子CXCL12及其受体CXCR4和CXCR7在HER-2过表达型乳腺癌和TNBC组织中高表达,可能与该类型乳腺癌预后较差有关,抑制其表达可为该类乳腺癌的治疗提供重要途径。
[Abstract]:Objective: to investigate the expression and clinical significance of chemokine CXCL12, its receptors CXCR4 and CXCR7 in different molecular types of breast cancer. Methods: Real-time quantitative PCR was used to detect chemokines in 80 cases of breast cancer with different molecular types. Expression of CXCL12 mRNA and its receptors CXCR4 and CXCR7 mRNA, Immunohistochemical technique was used to detect the expression of chemokine CXCL12 protein, its receptor CXCR4 and CXCR7 protein in 160 breast cancer cells with different molecular types. The results showed that there was no difference in the expression of CXCR4 and CXCR7 mRNA between Luminal A and Luminal B, and there was no difference in the expression of CXCR4 and CXCR7 mRNA in different molecular types of breast cancer. There was no difference in the expression of HER-2 overexpression type and triple negative breast cancer TNBCtype. However, the positive rates of the three proteins in HER-2 overexpression and TNBC were significantly higher than those in Luminal A and Luminal B (both P0.05CXCL12 and CXCR7 protein in HER-2 and TNBC were significantly higher than those in Luminal A and Luminal B (all P0.05A, P 0.05), but the positive rates of CXCR7 in Luminal A were significantly higher than those in Luminal A and Luminal B (both P0.05A and Luminal B). Conclusion: chemokine CXCL12 and its receptors CXCR4 and CXCR7 are highly expressed in HER-2 overexpressed breast cancer and TNBC tissues. It may be related to the poor prognosis of this type of breast cancer. Inhibition of its expression may provide an important approach for the treatment of this type of breast cancer.
【作者单位】：中南大学湘雅三医院乳腺甲状腺外科;
【基金】：湖南省科技厅科技计划项目(2013SK5074)~~
【分类号】：R737.9

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