Fra-1在肝癌中的表达及其与sAFP、血管侵犯的关系

发布时间：2018-03-15 02:16

本文选题：肝细胞癌　切入点：Fra-　出处：《安徽医科大学学报》2017年04期 　论文类型：期刊论文

【摘要】：目的检测Fra-1在肝细胞性肝癌(简称肝癌)及癌旁组织中的表达情况,探讨其与肝癌病理特征间的关系。方法采用免疫组化法检测Fra-1在60例肝癌及癌旁组织中的表达情况;采用Western blot、实时定量PCR(qRT-PCR)法检测Fra-1在20对冰冻肝癌及癌旁组织中的相对表达量。结果免疫组化示Fra-1在肝癌组织细胞核中阳性表达率为57%(34/60),细胞质中未见明显表达;而Fra-1在癌旁组织细胞质阳性表达率为23%(14/60),细胞核中未见明显表达。Fra-1在肝癌组织中的表达和血清甲胎蛋白(sAFP)、肿瘤血管侵犯相关(χ~2=4.538、9.086,P0.05),而与肝癌患者的肿瘤包膜、肝癌TNM分期、Edmondson分级等其他临床病理特征无明显相关性;Western blot及qRT-PCR检测结果示肝癌组织中Fra-1的相对表达量高于癌旁组织(P0.01)。结论 Fra-1与sAFP及肿瘤血管侵犯相关,提示Fra-1可能参与调控肝癌甲胎蛋白(AFP)的生成并影响肝癌的发展;为肝癌的诊治提供一些新的思路及理论基础。
[Abstract]:Objective to detect the expression of Fra-1 in hepatocellular carcinoma (HCC) and its adjacent tissues, and to explore the relationship between the expression of Fra-1 and the pathological features of HCC. Methods the expression of Fra-1 in 60 cases of HCC and its adjacent tissues was detected by immunohistochemical method. The relative expression of Fra-1 in 20 pairs of frozen liver cancer and its adjacent tissues was detected by Western blot.The results showed that the positive expression rate of Fra-1 in the nuclei of HCC tissues was 57% 34 / 60%, but no obvious expression was found in cytoplasm. However, the positive expression rate of Fra-1 in the cytoplasm of adjacent tissues was 23 / 14 / 60%. There was no significant expression of .Fra-1 in the nucleus of HCC tissues and serum alpha-fetoprotein (AFP) was correlated with tumor vascular invasion (蠂 ~ 2 / 2, 4.538 ~ 9.086 ~ (9.086) P 0.05), but it was associated with the tumor capsule of HCC patients. Other clinicopathological features such as TNM staging and Edmondson grade of hepatocellular carcinoma were not significantly correlated. The results of Western blot and qRT-PCR showed that the relative expression of Fra-1 in HCC tissues was higher than that in adjacent tissues (P 0.01). Conclusion Fra-1 is associated with sAFP and tumor vascular invasion. These results suggest that Fra-1 may be involved in the regulation of AFP production and affect the development of HCC, and provide some new ideas and theoretical basis for the diagnosis and treatment of HCC.
【作者单位】：安徽医科大学附属省立医院肝脏外科肝胆胰外科安徽省重点实验室;
【基金】：国家自然科学基金(编号:81272398) 安徽省公益性技术应用研究联动计划项目(编号:1604f0804011)
【分类号】：R735.7

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