Pin1与CyclinD1在胃肠间质瘤中的表达及临床意义

发布时间：2018-03-15 20:06

本文选题：胃肠间质瘤　切入点：Pin　出处：《中国医科大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的检测Pin1与Cyclin D1蛋白和m RNA在胃肠间质瘤(GIST)中的表达情况,探讨二者与GIST临床病理特征的相关性。方法分别采用免疫组化法和实时荧光定量PCR法检测Pin1与Cyclin D1蛋白和m RNA在GIST中的表达情况。结果Pin1和Cyclin D1蛋白在GIST中的表达率[64.7%(55/85),42.3%(36/85)]高于癌旁组织[26.7%(4/15),6.7%(1/15)];Pin1和Cyclin D1 m RNA在GIST组织中的表达量为癌旁组织中的7.03倍和5.53倍。Pin1蛋白及m RNA的表达与患者的临床病理参数无明显相关性,与GIST的NIH分级和肿瘤直径大小有关(均P0.05)。GIST组织中Pin1与Cyclin D1蛋白和m RNA的表达呈正相关。结论 Pin1与Cyclin D1蛋白和m RNA在GIST中表达增加,且二者的表达呈正相关,提示二者在GIST的发生发展中起协同作用。
[Abstract]:Objective to detect the expression of Pin1, Cyclin D1 protein and m RNA in gastrointestinal stromal tumors (GIST). Methods the expression of Pin1 and Cyclin D1 protein and m RNA in GIST were detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results Pin1 and Cyclin D1 protein were expressed in GIST. The expression of Pin1 and Cyclin D1 RNA in GIST tissues was 7.03 and 5.53 times higher than that in paracancerous tissues, and there was no significant correlation between the expression of m RNA and clinicopathologic parameters. There was a positive correlation between Pin1 and the expression of Cyclin D1 protein and m RNA in GIST. Conclusion the expression of Pin1 and Cyclin D1 protein and m RNA in GIST is increased, and the expression of Pin1 and Cyclin D1 protein is positively correlated with the expression of m RNA in GIST. The results suggest that the two play a synergistic role in the occurrence and development of GIST.
【作者单位】：福建医科大学附属泉州市第一医院消化内科;福建医科大学附属协和医院胃外科;惠安县医院普通外科;
【基金】：国家自然科学基金(81172380) 福建省卫生系统学术技术带头人后备人基金(010114201) 国家临床重点专科建设基金[卫办医政函(2012)649号]
【分类号】：R735

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