Wnt5a在肥胖合并肝细胞癌中表达的初步研究

发布时间：2018-03-16 08:40

本文选题：肥胖　切入点：肝细胞癌　出处：《四川大学学报(医学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨肥胖合并肝细胞癌中Wnt5a和β-连环蛋白(β-catenin)的表达情况。方法6周龄C57BL/6J小鼠分别给予普通饲料和高脂饲料喂养至14周龄(共8周),肥胖模型造模成功后采用尾静脉注射法将Hepa1-6肝癌细胞株(对照组注射生理盐水)分别接种于体质量正常小鼠和肥胖小鼠,18周龄时处死小鼠,眼眶取血分析相关血清学指标,麻醉下取肝组织后HE染色观察肝脏脂肪变性和肝细胞癌定植情况,免疫组化染色观察Wnt5a和β-catenin的表达情况。结果 18周龄时,高脂饲料喂养的肥胖小鼠肝脏细胞发生脂肪变性;接种肝癌细胞、体质量正常和肥胖小鼠肝组织中均查见肿瘤细胞团,在接种肝癌细胞的肥胖小鼠中可见肝组织灶状坏死。不同处理组小鼠血清总蛋白、天冬氨酸氨基转移酶、谷丙转氨酶、胆固醇和甲胎蛋白差异均有统计学意义(P0.05)。免疫组化染色结果显示,Wnt5a相对表达量为正常肝组织肥胖小鼠肝组织接种肝癌细胞后的体质量正常小鼠肝组织接种肝癌细胞后的肥胖小鼠肝组织(P0.05);β-catenin的相对表达量为接种肝癌细胞后的肥胖小鼠肝组织肥胖小鼠肝组织和接种肝癌细胞后的体质量正常小鼠肝组织正常肝组织(P0.05)。结论在肥胖小鼠中Wnt5a的表达下调,β-catenin异常蓄积,可能与肝细胞癌的形成和进展密切相关。
[Abstract]:Objective to investigate the expression of Wnt5a and 尾 -catenin in obese hepatocellular carcinoma (HCC). Methods C57BL / 6J mice aged 6 weeks were fed with normal diet and high fat diet to 14 weeks of age (8 weeks). Hepa1-6 hepatoma cells (control group) were inoculated with normal body weight mice and obese mice at the age of 18 weeks by tail vein injection, and the mice were killed at the age of 18 weeks. Serum samples were collected from the orbit, liver tissues were removed under anesthesia, liver steatosis and hepatocellular carcinoma colonization were observed by HE staining, and the expression of Wnt5a and 尾 -catenin were observed by immunohistochemical staining. Results at the age of 18 weeks, the expression of Wnt5a and 尾 -catenin were detected by HE staining. Fatty degeneration occurred in liver cells of obese mice fed with high fat diet and tumor cell mass was found in liver tissues of normal body mass and obese mice inoculated with hepatoma cells. Liver tissue necrosis was observed in obese mice inoculated with hepatoma cells. Serum total protein, aspartate aminotransferase, alanine aminotransferase were found in different treatment groups. The difference of cholesterol and alpha-fetoprotein was statistically significant (P 0.05). The results of immunohistochemical staining showed that the relative expression of Wnt5a was the same as that of normal liver tissue, obese mice, liver tissue inoculated with hepatoma cells, normal mice liver tissue inoculated with liver cancer fine. The relative expression of 尾 -catenin in the liver tissue of obese mice after inoculation with hepatoma cells and the normal liver tissue of mice inoculated with hepatoma cells was P0.050.Conclusion the relative expression of 尾 -catenin in the liver tissue of obese mice after inoculation of hepatoma cells and normal liver tissue of mice after inoculation of hepatoma cells is P0.05. The expression of Wnt5a was down-regulated and 尾 -catenin accumulated abnormally in mice. It may be closely related to the formation and progression of hepatocellular carcinoma.
【作者单位】：四川大学华西医院实验医学科;四川大学华西医院全科医学科;四川大学华西医院生物治疗国家重点实验室;四川大学华西医院内分泌代谢科;
【基金】：四川省科技厅科技支撑计划项目(No.2014SZ0170)资助
【分类号】：R735.7;R589.2

【参考文献】