磷脂酰肌醇通路相关分子Rictor及FoxO1与贲门癌关系的研究

发布时间：2018-03-17 01:02

本文选题：贲门癌　切入点：Rictor　出处：《河南科技大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:检测Rictor及Fox O1基因在贲门癌组织及对应癌旁组织中的表达差异,分析其与贲门癌临床病理特征之间的关系,判断其作为贲门癌早期诊断的可行性。方法:于2012年2月到2013年10月收集30例河南科技大学第一附属医院病理确诊并行贲门癌根治术的贲门癌患者,取其癌组织及对应癌旁组织,所有病例术前未经放疗和化疗。(1)贲门癌组织中Rictor及Fox O1基因的m RNA检测设计并合成了检测Rictor及Fox O1基因的引物,运用RT-PCR方法对贲门癌和对应癌旁组织中的Rictor及Fox O1 m RNA表达进行检测。(2)贲门癌组织中Rictor及Fox O1基因的蛋白检测运用免疫组化和Western blot的方法对贲门癌及其对应癌旁组织中Rictor及Fox O1基因的蛋白表达进行检测。运用SPSS17.0统计学软件对数据进行统计学处理,分析Rictor及Fox O1的表达与贲门癌患者的临床病理特征的关系。结果:(1)RT-PCR结果显示,在m RNA水平Rictor分子的表达在贲门癌组织及对应癌旁组织中均呈阳性,其中26例在贲门癌组织中的表达低于贲门癌对应癌旁组织中的表达(26/30),占86.7%,4例在贲门癌组织中的表达高于贲门癌相应癌旁组织中的表达(4/30),占13.3%,差异具有统计学意义(P0.05)。在m RNA水平Fox O1分子的表达在30例贲门癌组织及其对应癌旁组织中的表达均呈阳性,其中有25例在贲门癌组织中的表达低于贲门癌对应癌旁组织中的表达(25/30),占83.3%,5例在贲门癌组织中的表达高于对应癌旁组织中的表达(5/30),占16.7%,差异具有统计学意义(P0.05)。(2)免疫组化结果显示,在蛋白水平Rictor分子的表达在贲门癌组织及对应癌旁组织中的阳性率分别为46.7%(14/30)、76.7%(23/30),差异具有统计学意义(P0.05);相对于在对应癌旁组织中的表达,贲门癌组织中Rictor低表达与患者的年龄、性别、TNM分期及淋巴结转移均无关(P0.05),而与肿瘤分化程度密切相关(P0.05)。Fox O1蛋白在贲门癌组织、对应癌旁组织中的阳性率分别为53.3%(16/30)、86.7%(26/30),差异具有统计学意义(P0.05);相对于在对应癌旁组织中的表达,贲门癌组织中Fox O1低表达与患者的年龄、性别、肿瘤分化程度及淋巴结转移均无关(P0.05),与肿瘤TNM分期相关(P0.05)。(3)Western blot结果显示,Rictor蛋白在贲门癌组织及其对应癌旁组织中均呈阳性表达,其中24例在贲门癌组织中的表达低于贲门癌对应癌旁组织中的表达(24/30),占80.0%;6例在贲门癌组织的表达高于贲门癌对应癌旁组织中的表达(6/30),占20.0%,差异具有统计学意义(P0.05)。Fox O1蛋白在贲门癌组织及其对应癌旁组织中均呈阳性表达,其中24例在贲门癌组织中的表达低于贲门癌对应癌旁组织中的表达(24/30)占80.0%;6例在贲门癌组织中的表达高于贲门癌对应癌旁组织中的表达(6/30),占20.0%,差异具有统计学意义(P0.05)。(4)分别对Rictor和Fox O1的Western blot、RT-PCR结果进行一致性分析,结果为:r=0.609和r=0.429。说明Rictor和Fox O1蛋白表达和m RNA表达是一致的。(5)对Rictor和Fox O1进行相关性分析,Western blot及RT-PCR结果相关系数分别为:r=0.429和r=0.870。说明Rictor和Fox O1呈正相关。结论:(1)Rictor在贲门癌组织中的表达低于其在对应癌旁组织中的表达,提示Rictor可能参与了贲门癌发生、发展和侵袭转移的过程。(2)Fox O1在贲门癌组织中的表达低于其在对应癌旁组织中的表达,提示Fox O1可能与贲门癌的进程和转移相关,从侧面应证了贲门癌癌旁组织中Fox O1的高表达可能是贲门黏膜发生癌变的重要过程。(3)Rictor和Fox O1的表达呈正相关,可能是在PI3K/AKT/m TOR信号传导通路中,上游基因Rictor激活了下游基因Fox O1的表达,提示Rictor和Fox O1基因可能在贲门癌的发生发展中起着协同作用。
[Abstract]:Objective: to detect Rictor and Fox expression differences of O1 gene in gastric cancer tissues and corresponding adjacent to the analysis of its relationship with the clinicopathological features of cardiac carcinoma, as judge the feasibility of early diagnosis of cardiac cancer. Methods: from February 2012 to October 2013 collected 30 cases of the First Affiliated Hospital of Henan University of Science and Technology of pathological diagnosis of parallel radical resection of cardiac carcinoma in patients with cardia cancer, the cancer tissues and corresponding adjacent tissues, all cases without preoperative radiotherapy and chemotherapy. (1) m RNA Rictor and Fox O1 gene detection of cardiac carcinoma and synthesized primers to detect Rictor and Fox of O1 gene, using RT-PCR method to detect the expression of cardiac cancer and the tumor tissues of Rictor Fox and O1 m RNA. (2) was detected by immunohistochemistry and Western blot Rictor and Fox O1 gene in gastric cardiac carcinoma of cardia cancer and non cancerous To detect the expression of Rictor and Fox O1 gene proteins in the tissues. Using SPSS17.0 statistical software for statistical processing of data, the relationship between the clinicopathological features and expression analysis of Rictor Fox O1 and cardiac cancer patients. Results: (1) the results of RT-PCR showed that the expression level of RNA in M of Rictor molecules in the tissue of cardiac cancer and corresponding tumor tissues showed positive expression, the expression in 26 cases of cardiac carcinoma and cardia cancer below the tumor tissues (26/30), accounting for 86.7%, 4 cases expressed in cardiac carcinoma tissues was higher than that of cardia cancer adjacent tissue of the expression group (4/30), accounting for 13.3%, with statistical significant differences (P0.05). The expression of M RNA level Fox O1 molecule expression in 30 cases of cardia carcinoma and corresponding adjacent tissues were positive, the expression in 25 cases of cardiac carcinoma and cardia cancer below the tumor tissues (25/ 30), accounting for 83.3%, 5 cases of expression in gastric cardia carcinoma was higher than that of the corresponding tumor adjacent tissues (5/30), accounting for 16.7%, the difference was statistically significant (P0.05). (2) the results of immunohistochemistry showed that the positive rate of expression of Rictor at the protein level of molecules in cardiac cancer tissues and corresponding adjacent tissues were 46.7% (14/30), 76.7% (23/30), the difference was statistically significant (P0.05); compared with the expression in adjacent to the low expression of Rictor in gastric cardia carcinoma patients with age, gender, TNM stage and lymph node metastasis (P0.05), which is closely related with the tumor the degree of differentiation (P0.05) of.Fox O1 protein in cardiac carcinoma tissues, the positive rate of the tumor tissues were 53.3% (16/30), 86.7% (26/30), the difference was statistically significant (P0.05); relative to the organization in adjacent in the expression of Fox and low expression of O1 in patients with gastric cardia carcinoma age sex, The degree of tumor differentiation and lymph node metastasis (P0.05), associated with the stage of tumor TNM (P0.05). (3) Western blot results showed that the positive expression of Rictor protein in cardiac carcinoma tissues and corresponding adjacent tissues, the expression of the expression in 24 cases of cardiac carcinoma and cardia carcinoma adjacent below in the organization (24/30), accounting for 80%; the expression in 6 cases of cardiac cancer was higher than that of cardia cancer the tumor tissues (6/30), accounting for 20%, the difference was statistically significant (P0.05).Fox O1 protein group were positive in the fabric in the tissue of cardiac cancer and corresponding para carcinoma, the expression of expression in 24 cases of cardiac carcinoma and cardia cancer below the tumor tissues (24/30) accounted for 80%; the expression in 6 cases of cardiac carcinoma and cardia cancer than the tumor tissues (6/30), accounting for 20%, the difference was statistically significant (P0.05). (4) respectively Rictor and F Ox O1 Western blot RT-PCR, the consistency analysis, results are as follows: r=0.609 and r=0.429. Rictor and expression of Fox O1 protein and M Expression of RNA is consistent. (5) to analyze the correlation between the Rictor and Fox O1, Western blot and RT-PCR the correlation coefficient were r= 0.429 and r=0.870. Rictor and positively Fox O1. Conclusion: (1) the expression of Rictor in gastric cardia carcinoma tissues than that in adjacent tissues and the expression of Rictor may be involved in the process of cardia cancer, development and metastasis of Fox. (2) the expression of O1 in gastric cardia carcinoma is lower than its expression in adjacent tissues the Fox O1 may be associated with cardiac carcinoma and metastasis process, from the side should permit a high expression of gastric cardia adenocarcinoma tissues Fox O1 may be an important process in the cardia mucosa carcinogenesis. (3) the expression of Rictor was positively related to O1 and Fox, may be in P In the I3K/AKT/m TOR signaling pathway, the upstream gene Rictor activated the downstream gene Fox O1 expression, suggesting that Rictor and Fox O1 gene may play a synergistic role in the occurrence and development of gastric cardia cancer.

【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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