Survivin和Ki-67在桥本氏甲状腺炎合并甲状腺乳头状癌中的表达及意义

发布时间：2018-03-17 01:11

本文选题：桥本氏甲状腺炎　切入点：甲状腺乳头状癌　出处：《扬州大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨Survivin和Ki-67在桥本氏甲状腺炎(Hashimoto's Thyroiditis, HT)、甲状腺乳头状癌(Papillary Thyroid Carcinoma, PTC)、及桥本氏甲状腺炎合并甲状腺乳头状癌中的表达及临床病理意义。方法采用免疫组化SP法检测140例甲状腺标本中Survivi n和Ki-67蛋白表达情况,其中甲状腺腺瘤20例、HT标本40例、PTC标本40例、HT合并PTC标本40例。结果1、Survivin蛋白表达在甲状腺腺瘤组织、HT、PTC; HT合并PTC组织中的阳性率分别为10%(2/20)、42.5%(17/40)、77.5%(31/40)、72.5%(29/40)；HT组(χ2=6.508,p=0.011)、PTC组(χ2=24.545,p=0.000)及HT合并PTC组(χ2=20.857,p=0.000)中Survivin蛋白表达阳性率均明显高于甲状腺腺瘤组,HT合并PTC中Survivin蛋白的表达也显著高于HT组(χ2=7.366,p=0.007),而HT合并PTC组与PTC组比较差异无统计学意义(p0.05)。2、临床资料分析显示Survivin蛋白在HT合并PTC中的表达与临床分期(χ2=4.477,p=0.03)及淋巴转移(χ2=6.930,p=0.008)相关,而与年龄(χ2=0.189,p=0.664)及性别(χ2=0.005,p=0.944)没有明显关联。3、Ki-67蛋白表达在甲状腺腺瘤组织、HT、PTC、HT合并PTC组织中的阳性率分别为15%(3/20)、57.5%(23/40)、67.5%(27/40)、65%(26/40)；HT组(χ2=9.808,p=0.002)、PTC组(χ2=14.700,p=0.000)及HT合并PTC组(χ2=13.348,p=0.000)中Ki-67蛋白表达阳性率均显著高于甲状腺腺瘤组织,HT合并PTC组中Ki-67蛋白表达阳性率与HT组(p0.05)及PTC组(p0.05)比较差异无统计学意义。4、临床资料分析显示,HT合并PTC组中Ki-67蛋白的表达与临床分期(x2=4.062,p=0.044)及淋巴转移(χ2=7.016,p=0.008)相关,而与年龄(χ2=0.101,p=0.75)及性另(x2=0.23,p=0.631)没有明显关联。5、在HT合并PTC组织中,Survivin和Ki-67的阳性表达呈正相关(r=0.146,p=0.007)。结论HT与PTC之间关系密切,HT具有一定的癌变倾向,HT可能为HT合并PTC的癌前病变,而Survivin和Ki-67的异常高表达可能在HT合并PTC的发生、发展、侵袭及远处转移等过程中起着重要的作用。
[Abstract]:Objective to investigate the expression and clinicopathological significance of Survivin and Ki-67 in Hashimoto's thyroiditis Thyroiditis, Thyroiditis, papillary Thyroid Carcinoma, papillary thyroid carcinoma, and Hashimoto's thyroiditis complicated with thyroid papillary carcinoma. Methods Immunohistochemical SP method was used to detect the expression and clinicopathological significance of Survivin and Ki-67 in patients with thyroid papillary carcinoma. The expression of Survivi n and Ki-67 protein in 140 thyroid specimens. Results 1 the positive rate of survivin protein expression in thyroid adenoma tissues was 102 / 20 / 42.5 / 42.5 / 77.5A = 31 / 4072.5% / 29 / 29 / 40 ~ (40) T (蠂 ~ 26.508p 0.011p0.000), respectively (蠂 ~ 26.508p 0.011p0.000) and HT + PTC (224.857p0.000), respectively. The positive rate of survivin protein expression in thyroid adenoma tissues was 102.20% 42.5% ~ 77.55% (蠂 ~ 2 6.508p0.011p0.000) and that of HT combined with PTC was 102.20% ~ 47.55% respectively (蠂 ~ -6.508p 0.011p0.000), and that of HT combined with PTC was 107.55% (蠂 ~ 26.508p 0.011p0.000). The positive rate of Survivin protein expression in HT + PTC was significantly higher than that in HT + PTC in thyroid adenoma group (蠂 ~ 2 / 7.366p ~ (0.007) P ~ (0.007)), but there was no significant difference between HT + PTC group and PTC group (P < 0.05). The clinical data analysis showed that Survivin protein was not significantly different from that in HT + PTC group. The expression of HT combined with PTC was correlated with clinical stage (蠂 2 / 4.477) and lymphatic metastasis (蠂 2 / 6.930 / P 0.008). However, there was no significant correlation between age (蠂 2 / 0.189) and sex (蠂 2 / 0.005 / P ~ (0.944)). The positive rate of Ki-67 protein expression in thyroid adenoma tissues with PTC was 15 ~ 320% ~ 57.5%, respectively. The positive rate of Ki-67 protein expression in HT group was significantly higher than that in HT group (蠂 ~ 29.808) and HT combined with PTC group (213.348p0.000), respectively. The positive rate of expression of Ki-67 protein was significantly higher in HT group (蠂 29.808) and HT combined with PTC group (213.348p0.000) than in the control group (蠂 29.808) and HT combined with PTC group (213.348p0.000), respectively. The positive rate of the expression of Ki-67 protein was significantly higher than that in the control group (蠂 29.808) and the HT combined with PTC group (213.348p0.000), respectively. The positive rate of the expression of Ki-67 protein was significantly higher than that in the control group. The positive rate of Ki-67 protein expression in adenoma tissues with PTC was not significantly different from that in HT group (p0.05) and PTC group (p0.05). The clinical data analysis showed that the expression of Ki-67 protein was correlated with the clinical stage and lymph metastasis (蠂 27.016p0.008). However, there was no significant correlation with age (蠂 ~ 2 + 0.101) and sex (X ~ (2 +) = 0.23 ~ (0.23) P ~ (0.63)). There was a positive correlation between the expression of survivin and Ki-67 in HT with PTC. Conclusion there is a close relationship between HT and PTC. HT may be the precancerous lesion of HT combined with PTC. The abnormal overexpression of Survivin and Ki-67 may play an important role in the occurrence, development, invasion and distant metastasis of HT associated with PTC.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R736.1;R581.4

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