儿茶酚氧位甲基转移酶在结直肠腺瘤及癌组织中的表达及其与结直肠癌临床病理性质的相关性

发布时间：2018-03-19 07:00

本文选题：结直肠腺瘤　切入点：结直肠癌　出处：《北京协和医学院》2015年博士论文　论文类型：学位论文

【摘要】：目的：研究儿茶酚氧位甲基转移酶(COMT)在结直肠腺瘤及癌组织中的动态表达情况及其与结直肠癌(CRC)临床病理性质的相关性。方法：留取2013年1月至11月诊治的18例结直肠腺瘤病例的新鲜腺瘤组织及瘤旁正常黏膜,提取RNA,进行逆转录定量PCR。留取2013年1月至3月诊治的49例结直肠腺瘤病例的新鲜腺瘤组织及瘤旁正常黏膜组织,制成切片,进行免疫组化染色。留取2014年7月至8月诊治的15例CRC病例的新鲜癌组织及癌旁正常黏膜组织,提取RNA,进行逆转录定量PCR。筛选2012年6月至9月诊治的42例CRC病例入组,将冻存的癌组织及癌旁正常黏膜制成切片,进行免疫组化染色(其中1例同时进行逆转录定量PCR)。筛选2007至2009年诊治的81例CRC病例入组,查找病理科存放的相应组织蜡块并制片,进行免疫组化染色。得到数据使用SPSS 13.0进行配对t检验或独立t检验进行统计学分析。结果：18例进行逆转录定量PCR的结直肠腺瘤病例中,编码COMT的nRNA在腺瘤组织中的相对表达量明显高于瘤旁正常对照组织,CI 95%,P=0.011。49例进行免疫组化染色评分的结直肠腺瘤病例中,COMT蛋白在腺瘤组织中的表达明显高于瘤旁正常对照组织,CI 95%,P=0.000。16例进行逆转录定量PCR的CRC病例中,编码COMT的mRNA在癌组织中的相对表达量明显高于癌旁正常对照组织,CI95%,P=0.007。42例进行免疫组化染色评分的CRC病例中,COMT蛋白在癌组织中的表达明显高于癌旁正常对照组织,CI 95%,P=0.000。进行免疫组化的81例CRC病例中,COMT蛋白在早期癌组织中的表达明显高于进展期癌组织,对于T/N/TNM, CI 95%, P=0.018/P=0.000/P=0.000,而早期癌组织中COMT蛋白的表达与腺瘤组织未发现明显差异,对于T/N/TNM, CI 95%, P=0.933/P=0.527/P=0.227;另外,COMT蛋白在高、中分化癌组织中的表达明显高于低分化癌组织,CI 95%,P=0.005,而高/中分化癌组织中COMT蛋白的表达与腺瘤组织未发现明显差异,CI 95%,P=0.153。结论：COMT与结直肠腺瘤及癌的发生存在相关性,与CRC进展过程及去分化过程存在相关性,具有作为CRC诊断、分期、分化程度的参考指标的潜力。
[Abstract]:Objective: to study the dynamic expression of catechol-oxomethyltransferase (COMT) in colorectal adenoma and carcinoma and its correlation with clinicopathological properties of colorectal carcinoma. Methods: 18 patients with colorectal carcinoma from January 2013 to November were enrolled in this study. The fresh adenoma tissue and adjacent normal mucosa of colorectal adenoma. RNA was extracted from 49 cases of colorectal adenoma treated from January 2013 to March. The fresh adenoma tissue and normal mucosa adjacent to the tumor were collected and made into sections. From July 2014 to August, 15 cases of CRC were collected from the fresh cancer tissue and normal mucosa adjacent to the tumor, and then the RNA was extracted, and then reverse transcription quantitative PCR was performed. 42 cases of CRC from June 2012 to August were selected into the group. Frozen cancer tissue and normal mucosa adjacent to carcinoma were made into sections, and immunohistochemical staining was performed in one case. 81 cases of CRC diagnosed and treated from 2007 to 2009 were selected for examination and examination of corresponding tissue wax pieces stored in pathology department. SPSS 13.0 was used for paired t test or independent t test for statistical analysis. Results in 18 cases of colorectal adenoma with reverse transcription quantitative PCR, The relative expression of nRNA encoding COMT in adenoma tissue was significantly higher than that in adjacent normal control group (P0.011.49 cases with colorectal adenoma by immunohistochemical staining). In the normal control tissue, CIC95 / P was 0.000.16 cases of CRC with reverse transcription quantitative PCR. The relative expression of mRNA encoding COMT in cancer tissue was significantly higher than that in adjacent normal control group (P0.007.42 CRC cases), which was significantly higher than that in adjacent normal control group (P0.007.42) (P = 0.000). The expression of CRC protein in the early stage of cancer was significantly higher than that in the advanced stage of cancer, which was detected by immunohistochemistry in 81 cases of CRC. For T / N / TNM, CI95, P0. 018 / P0. 000 / P0. 000, there was no significant difference between the expression of COMT protein in early cancer tissue and adenoma tissue. For T / N / TNM, CI95, P0. 933 / P0. 527 / P0. 227; in addition, COMT protein was high. The expression of COMT protein in moderately differentiated carcinoma tissues was significantly higher than that in poorly differentiated carcinoma tissues. However, there was no significant difference between the expression of COMT protein and adenoma tissues. Conclusion there is a correlation between the expression of COMT protein and colorectal adenoma and carcinogenesis. It has the potential to be used as a reference index for the diagnosis, staging and differentiation of CRC.
【学位授予单位】：北京协和医学院
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R735.34

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