肝癌组织中LUZP表达变化及其与患者临床病理特征的关系

发布时间：2018-03-20 02:31

本文选题：肝细胞癌　切入点：亮氨酸拉链蛋白　出处：《山东大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:目的探讨亮氨酸拉链蛋白(LUZP)表达与肝癌患者临床病理特征的关系。方法:选择接受手术治疗的77例肝癌患者的肝癌及癌旁肝组织,采用实时定量PCR法检测癌及癌旁肝组织中LUZP mRNA的表达,采用免疫组化法检测癌及癌旁肝组织中LUZP蛋白的表达,半定量计分法对免疫组化结果进行统计分析。以癌旁肝组织LUZP mRNA均值为标准,将肝癌患者分为LUZP高表达、低表达两个水平,比较不同LUZP表达水平患者的临床病理特征。结果:肝癌与癌旁肝组织LUZP mRNA表达分别为1.87±0.11、0.58±0.05,肝癌组织中LUZP mRNA表达高于癌旁肝组织(P0.05)。肝癌与癌旁肝组织LUZP蛋白表达分别为(10.0±1.5)、(4.0士0.5)分,肝癌组织中LUZP蛋白表达高于癌旁肝组织(P0.05)。以癌旁肝组织LUZP mRNA均值为标准,将肝癌患者分为LUZP高表达49例、低表达28例。LUZP高表达患者侵犯门静脉、TNM 1+2期、复发的发生率均高于低表达患者(P均0.05)。低表达患者的总生存时间是36个月,高表达患者的总生存时间是12个月,LUZP高表达患者的生存时间低于低表达患者(P0.05,OR=1.88,95%CI为1.08～3.30)。结论:LUZP是肝癌患者预后的生物学指标,其高表达提示肝癌患者预后差。
[Abstract]:Objective: to investigate the relationship between the expression of leucine zipper protein (LUZP) and the clinicopathological features of hepatocellular carcinoma (HCC). The expression of LUZP mRNA in cancer and adjacent liver tissues was detected by real-time quantitative PCR, and the expression of LUZP protein was detected by immunohistochemistry. The results of immunohistochemistry were statistically analyzed by semi-quantitative scoring method. According to the mean value of LUZP mRNA in adjacent liver tissues, the patients with liver cancer were divided into two levels: high expression of LUZP and low expression of LUZP. Results: the expression of LUZP mRNA was 1.87 卤0.110.58 卤0.05 in hepatocellular carcinoma and paracancerous liver tissues, respectively. The expression of LUZP mRNA in HCC was higher than that in paracancerous liver tissue (P0.05A). The expression of LUZP protein in HCC and paracancerous liver tissues was 10.0 卤1.5 + 4.0 卤0.5, respectively. The expression of LUZP protein in HCC tissue was higher than that in paracancerous liver tissue (P0.05). According to the mean value of LUZP mRNA in paracancerous liver tissue, 49 cases of HCC were classified as high expression of LUZP and 28 cases of low expression of LUZP were involved in portal vein TNM12 stage. The incidence of recurrence was higher in patients with low expression than that in patients with low expression (P < 0.05). The total survival time of patients with low expression was 36 months. The total survival time of patients with high expression was 12 months. The survival time of patients with high expression of LUZP was lower than that of patients with low expression of LUZP. Conclusion the survival time of patients with high expression of LUZP is lower than that of patients with low expression of LUZP. Conclusion the survival time of patients with high expression of LUZP is lower than that of patients with low expression of LUZP.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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