葡萄籽原花青素对肺癌A549细胞侵袭和迁移的影响及其机制

发布时间：2018-03-20 03:19

本文选题：葡萄籽原花青素　切入点：A549细胞　出处：《重庆医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:探讨葡萄籽原花青素(GSP)对肺癌A549细胞侵袭、迁移能力的影响及其机制。方法:用台盼蓝(Trypan Blue)法检测不同浓GSP对BEAS-2B人正常肺上皮细胞处理后的的毒性作用,利用(0、10、20、40、80)μg/mL GSP处理细胞,利用MTT法测定肺癌A549细胞在体外情况下的增殖水平;利用Transwell?实验检测肺癌A549细胞在体外情况下的侵袭力;利用细胞划痕实验检测肺癌A549细胞在体外情况下的迁移力;利用Western blot法测定肺癌A549细胞中各类肿瘤相关抗体表皮生长因子受体(EGFR)、上皮型钙黏素,也有文献称之为E型黏钙蛋白(E-cadherin)、神经型钙黏素,也有文献称之为N型黏钙蛋白(N-cadherin)的蛋白水平。结果:不同浓度梯度的(0、10、20、40、)μg/mL GSP对BEAS-2B正常肺上皮细胞无明显毒性作用,80μg/mLGSP毒性作用明显。GSP以剂量依赖的方式抑制A549细胞的增殖;实验组的肺癌A549细胞侵袭和迁移能力均显著下降;相比较对照组而言,Western blot结果显示经过GSP作用处理后的肺癌A549细胞,其蛋白表达量EGFR和N-cadherin蛋白降低,而E-cadherin蛋白升高。结论葡萄籽原花青素(GSP)对正常肺上皮细胞药物副作用,药物毒性都十分小,并且调节控制了肺癌A549细胞中EGFR和N-cadherin及E-cadherin的表达,其抑制A549细胞的增殖、侵袭和迁移的机制就可能与之存在密切关系。
[Abstract]:Objective: to investigate the effect of grape seed procyanidin (GSP) on the invasion and migration of lung cancer A549 cells and its mechanism. Methods: the toxic effects of different concentrations of GSP on BEAS-2B human normal pulmonary epithelial cells were detected by trypan blue blue method. The proliferation of lung cancer A549 cells in vitro was measured by MTT assay, and the cell proliferation was measured by MTT method. The invasiveness of lung cancer A549 cells in vitro and the migration of lung cancer A549 cells in vitro were detected by cell scratch assay. Western blot assay was used to determine the expression of EGFR receptor, E-cadherin, neurotype cadherin and E-cadherin in human lung cancer cell line A549, and the expression of E-cadherin in human lung cancer A549 cells was determined by using the method of EGF receptor and E-cadherin, which was also called E-cadherin in human lung cancer cell line A549. Results: there was no obvious toxic effect of different concentration gradient of MLGSP on BEAS-2B normal pulmonary epithelial cells. GSP inhibited the proliferation of A549 cells in a dose-dependent manner. Compared with the control group, the expression of EGFR and N-cadherin protein in A549 cells treated with GSP was decreased. Conclusion GSPs can inhibit the proliferation of A549 cells by regulating the expression of EGFR, N-cadherin and E-cadherin in A549 cells. The mechanism of invasion and migration may be closely related to it.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R734.2

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