抑瘤素M通过诱导衰老抑制肝癌细胞增殖

发布时间：2018-03-21 06:48

本文选题：肝癌细胞　切入点：抑瘤素M　出处：《中国肿瘤临床》2017年08期 　论文类型：期刊论文

【摘要】：目的:通过分析抑瘤素M(oncostatin M,OSM)对肝癌细胞生长的效应,研究其影响细胞增殖的分子机制。方法:OSM处理SMMC-7721和Hep G2肝癌细胞系,观察细胞的增殖速率和形态变化,结合特异性β-半乳糖苷酶染色和细胞周期分析,研究OSM是否通过诱导肝癌细胞进入衰老状态来抑制其增殖;进一步通过监测细胞周期抑制蛋白p16、p21、p27和癌基因c-Myc的表达变化,分析OSM诱导细胞衰老的原因。结果:OSM可以抑制肝癌细胞系生长,且抑制率呈现一定剂量依赖性;细胞形态变化和β-半乳糖苷酶染色进一步证实OSM可诱导细胞衰老。细胞周期分析表明OSM阻滞肝癌细胞于G0/G1期,并伴随p21和p27周期抑制蛋白的表达增高。最后,通过分析STAT3信号途径下游癌基因c-Myc的转录与蛋白水平,表明OSM可能是通过癌基因的激活而诱导细胞的衰老。结论:由癌基因激活而导致的细胞衰老,是机体的一种防御机制。OSM通过激活STAT3信号途径、上调癌基因cMyc表达的同时,也加速了细胞的衰老,从而最终表现为对肝癌细胞增殖的抑制作用。
[Abstract]:Objective: to study the effect of Mantoncostatin on the growth of hepatoma cells and its molecular mechanism. Methods: SMMC-7721 and Hep G2 hepatoma cell lines were treated with SMMC-7721 and Hep, and the proliferation rate and morphology of the cells were observed. Combined with specific 尾 -galactosidase staining and cell cycle analysis, we studied whether OSM inhibits the proliferation of hepatocellular carcinoma cells by inducing them to enter the aging state, and further monitored the expression of cell cycle suppressor protein p16, p21, p27 and oncogene c-Myc. Results OSM could inhibit the growth of hepatoma cell line in a dose-dependent manner. Cell morphological changes and 尾 -galactosidase staining further confirmed that OSM could induce cell senescence. Cell cycle analysis showed that OSM blocked hepatoma cells at G _ 0 / G _ 1 phase, accompanied by increased expression of p21 and p27 cycle suppressor proteins. By analyzing the transcription and protein levels of oncogene c-Myc in the downstream of STAT3 signaling pathway, it is suggested that OSM may induce cell senescence through the activation of oncogene. OSM upregulated the expression of oncogene cMyc and accelerated cell senescence by activating STAT3 signaling pathway, which resulted in the inhibition of hepatoma cell proliferation.
【作者单位】：天津医科大学三中心临床学院;天津市三中心医院天津市肝胆疾病研究所;天津市人工细胞重点实验室;卫生部人工细胞工程技术研究中心;
【基金】：天津市科委应用基础与前言技术研究计划项目(编号:15JCQNJC45700,15JCQNJC11500) 天津市卫生行业重点攻关项目(编号:12KG107,12KG108)资助~~
【分类号】：R735.7

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