木鳖子单体化合物对羟基桂皮醛对食管癌移植瘤的抑制作用

发布时间：2018-03-23 00:25

本文选题：木鳖子单体化合物对羟基桂皮醛　切入点：食管癌　出处：《中国肿瘤生物治疗杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨木鳖子单体化合物对羟基桂皮醛(p-hydroxylcinnamaldehyde,CMSP)对食管癌细胞增殖及在BALB/c裸鼠移植瘤生长的抑制作用。方法:细胞计数法检测CMSP对食管癌细胞株Kyse 30和TE-13增殖的抑制作用,RT-PCR法检测食管癌细胞中CEA和SCC mRNA的表达情况,Western blotting法检测食管癌细胞中C-myc和N-myc蛋白的表达变化。构建裸鼠食管癌细胞移植瘤模型,分为对照组和CMSP处理组,实验结束后测量移植瘤大小及体积;免疫组织化学SP法观察移植瘤组织C-myc和N-myc蛋白的表达变化,H-E染色后光镜下观察两组裸鼠移植瘤组织和肝、脾组织的病理学变化。结果:CMSP对食管癌细胞Kyse 30和TE-13的增殖具有明显的抑制作用[(1.7±0.3)vs(3.8±0.3),(1.6±0.2)vs(4.5±0.4),均P0.01]。经CMSP处理后:(1)Kyse 30和TE-13细胞中CEA和SCC mRNA表达降低(P0.01)、C-myc和N-myc蛋白表达明显低于对照组(P0.05或P0.01);(2)裸鼠移植瘤平均体积和质量明显降低(P0.05或P0.01),移植瘤组织中Cmyc和N-myc蛋白表达水平低于盐水对照组肿瘤组织。CMSP处理组和对照组中肝、脾组织并未发生任何病理改变。结论:木鳖子单体化合物CMSP体外抑制食管癌细胞增殖,体内抑制裸鼠食管癌移植瘤的生长。
[Abstract]:Objective: to investigate the inhibitory effect of monomeric compound of Trionyx chinensis on the proliferation of esophageal carcinoma cells and the growth of transplanted tumor in BALB/c nude mice. Methods: the inhibitory effect of CMSP on the proliferation of esophageal carcinoma cell lines Kyse 30 and TE-13 was detected by cell count method. The expression of CEA and SCC mRNA in esophageal carcinoma cells was detected by RT-PCR and the expression of C-myc and N-myc in esophageal carcinoma cells was detected by Western blotting. At the end of the experiment, the size and volume of transplanted tumor were measured, the expression of C-myc and N-myc protein in transplanted tumor tissues were observed by immunohistochemical SP method, and the transplanted tumor tissues and liver of two groups were observed under light microscope after H-E staining. Results the proliferation of Kyse 30 and TE-13 in esophageal carcinoma cells was significantly inhibited by CMSP treatment [1.7 卤0.3)vs(3.8 卤0.3 卤0.4, respectively, P0.01]. After CMSP treatment, the expression of CEA and SCC mRNA was significantly lower than that of P0.01C-myc and N-myc. The mean volume and quality of transplanted tumor in nude mice were significantly lower than those in control group (P 0.05 or P 0.01). The expression level of Cmyc and N-myc protein in transplanted tumor tissue was lower than that in saline control group. Conclusion: CMSP can inhibit the proliferation of esophageal carcinoma cells in vitro and inhibit the growth of transplanted tumor of esophageal carcinoma in nude mice in vivo.
【作者单位】：河北医科大学第四医院河北省肿瘤基因诊断预防和治疗重点实验室;河北医科大学第四医院科研中心;
【基金】：国家自然科学基金青年基金资助项目(No.81502032) 河北省医学科学研究重点课题计划资助项目(No.20120120)~~
【分类号】：R735.1

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