Akt和SRC在Exosomes促进同源肺癌细胞迁移中的作用

发布时间：2018-03-23 03:22

本文选题：Exosomes　切入点：迁移　出处：《中国医科大学学报》2017年04期 　论文类型：期刊论文

【摘要】：目的研究肺癌细胞来源的Exosomes对其分泌细胞及同源肿瘤细胞迁移的影响,初步探讨PI3K/Akt和SRC信号转导通路在此过程中的作用。方法采用密度梯度离心法,从肺癌细胞A549的上清液中分离出肺癌细胞来源的Exosomes,利用透射电子显微镜观察Exosomes的形态,用Western blotting实验观察蛋白的表达。Transwell实验检测细胞的迁移能力。结果透射电子显微镜下观察肺癌A549细胞来源的Exosomes,其具有特征性的盘状结构,由双层膜构成,直径30~100 nm。Western blotting结果显示Exosomes表面富含CD9分子。A549细胞来源的Exosomes以剂量依赖性的方式促进A549和其同源肿瘤细胞HCC827细胞的迁移,并伴随Akt和SRC的活化。结论肺癌细胞来源的Exosomes能促进其分泌细胞和同源肿瘤细胞的迁移,其机制可能与Akt和SRC的活化有关。
[Abstract]:Objective to study the effect of Exosomes from lung cancer cells on the migration of secreting cells and tumor cells, and to explore the role of PI3K/Akt and SRC signal transduction pathway in the process. Methods density gradient centrifugation was used. Exosomes, a lung cancer cell line, was isolated from the supernatant of lung cancer cell A549. The morphology of Exosomes was observed by transmission electron microscope (TEM). Western blotting assay was used to observe the expression of protein. Transwell assay was used to detect the migration ability of A549 cells. Results Exosomes from lung cancer A549 cells was observed under transmission electron microscope, which had a characteristic disk-like structure and was composed of bilayer membrane. The results of 30 ~ 100 nm.Western blotting showed that Exosomes, which was rich in CD9 molecule. A549 cells on the surface of Exosomes, promoted the migration of A549 and its homologous tumor cells HCC827 cells in a dose-dependent manner. Conclusion Exosomes derived from lung cancer cells can promote the migration of secretory cells and homologous tumor cells, and its mechanism may be related to the activation of Akt and SRC.
【作者单位】：中国医科大学附属第一医院呼吸疾病研究所老年病呼吸感染科;中国医科大学附属第一医院肿瘤内科辽宁省抗肿瘤药物与生物治疗重点实验室;中国医科大学附属第一医院呼吸疾病研究所呼吸内科;
【基金】：国家自然科学基金(81372546,81472193) 辽宁省科学技术计划(2014226033,2014225013,L2014296) 辽宁省教育厅重点实验室基础研究(LZ2014037)
【分类号】：R734.2

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