星形细胞上调基因-1与钙黏附蛋白-E在食管癌中表达的相关性及其意义

发布时间：2018-03-23 04:27

本文选题：星形细胞上调基因-1　切入点：钙黏附蛋白-E　出处：《新乡医学院》2016年硕士论文　论文类型：学位论文

【摘要】：背景食管癌是我国常见消化道恶性肿瘤,由于早期症状不典型,多数患者发现时已属晚期,因此具有较高的死亡率,居全国各类恶性肿瘤的第4位,其中深度浸润和转移是主要的死因。目前对于食管癌的治疗除传统的外科手术、放化疗治疗外,随着人们对肿瘤发病的分子水平的研究不断加深,针对新分子靶点的分子靶向治疗成为食管癌治疗的新方向。星形细胞上调基因-1(astrocyte elevated gene-1,AEG-1)是新近发现的癌基因。研究发现AEG-1在多种肿瘤(如乳腺癌、神经胶质瘤、前列腺癌、胃癌等)中存在高表达,并与肿瘤的进展、转移与侵袭、不良预后密切相关。钙黏附蛋白-E(E-cadherin,E-ca)是表达于正常上皮细胞表面的黏附分子,介导上皮细胞间的黏附性。E-cadherin在多种肿瘤中存在明显表达减少甚至缺失,与肿瘤的转移与侵袭、不良预后密切相关。然而两者在食管癌中表达的相关性并未见报道。该研究拟通过免疫组织化学和Western blot检测食管癌及相应癌旁食管黏膜中AEG-1和E-ca表达水平,并分析两者与临床病理特征的关系,探讨两者在食管癌中表达的相关性及其意义。目的研究AEG-1和E-ca在食管癌中的表达的相关性,并分析与临床病理特征的关系。初步探讨两者在食管癌浸润、转移中的作用。方法采用免疫组化方法和Western blot两种方法检测58例食管癌组织及其相应的癌旁组织中AEG-1和钙黏附蛋白-E的表达;并收集患者的完整病理资料。分析两者与性别、年龄、肿瘤大小、分化程度、淋巴结转移、临床分期之间的相关性;应用SPSS19.0统计软件,计数资料采用χ2检验,相关性采用Spearman相关分析,P0.05为差异有统计学意义。结果1.在58例食管癌组织中AEG-1表达的阳性率82.7%明显高于相应的癌旁组织的51.7%(P0.05),且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型不相关(P0.05),而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关(P0.05)。2.在58例食管癌组织中钙黏附蛋白-E的阳性率为31.0%,明显低于相应癌旁组织的94.8%(P0.05),且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型不相关(P0.05),而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关(P0.05)。3.食管癌中AEG-1与E-ca的表达呈明显的负相关(r=-0.483,P0.05)。结论1.AEG-1和E-ca共同参与食管癌的侵润转移过程,联合检测AEG-1和E-ca是判断食管癌患者转移潜能和预后的重要因素。2.AEG-1可能介导食管癌中E-ca的表达下调。
[Abstract]:Background esophageal cancer is a common malignant tumor of digestive tract in China. Because of the atypical early symptoms, most of the patients were found to be advanced, so they have a high mortality rate, ranking fourth among all kinds of malignant tumors in China. Among them, deep infiltration and metastasis are the main causes of death. At present, the treatment of esophageal cancer, in addition to traditional surgery, radiotherapy and chemotherapy, with the deepening of the study of the molecular level of tumor, Molecular targeting therapy for new molecular targets has become a new direction in the treatment of esophageal cancer. The astrocyte up-regulation gene elevated gene-1 AEG-1 is a newly discovered oncogene. AEG-1 has been found in many tumors (such as breast cancer, glioma, prostate cancer). High expression was found in gastric cancer, and was closely related to tumor progression, metastasis, invasion and poor prognosis. Calcitonin E-cadherin E-cac is a adhesion molecule expressed on the surface of normal epithelial cells. The expression of E-cadherin, mediated by adhesion between epithelial cells, is reduced or even absent in many kinds of tumors, and it is associated with the metastasis and invasion of tumor. The expression of AEG-1 and E-ca in esophageal carcinoma and its adjacent esophageal mucosa were detected by immunohistochemistry and Western blot. To study the relationship between the expression of AEG-1 and E-ca in esophageal carcinoma, and the relationship between them and clinicopathological features, and to explore the relationship between the expression of AEG-1 and E-ca in esophageal carcinoma. The relationship between them and clinicopathological features was analyzed. Methods Immunohistochemical method and Western blot were used to detect the expression of AEG-1 and cadherin E in 58 cases of esophageal carcinoma and their adjacent tissues, and the complete pathological data of the patients were collected. The correlation between age, tumor size, differentiation degree, lymph node metastasis, clinical stage, SPSS19.0 statistical software, count data by 蠂 2 test, Results 1. The positive rate of AEG-1 expression in 58 cases of esophageal carcinoma was significantly higher than that of the corresponding adjacent tissues (51.7%), and the positive rate of AEG-1 expression was significantly higher than that of the corresponding adjacent tissues (51.7%), and the positive rate was related to sex, age and location of tumor. 2. The pathological type of the tumor was not related to P0.05, but to the degree of differentiation, depth of invasion and clinical stage. The positive rate of cadherin E in 58 cases of esophageal carcinoma was 31.0, which was significantly lower than that of the corresponding adjacent tissues (P 0.05), and the positive rate was related to sex, age and location of tumor. The expression of AEG-1 and E-ca in esophageal carcinoma was negatively correlated with the degree of differentiation, depth of invasion, clinical stage and lymph node metastasis. Conclusion 1.AEG-1 and E-ca are involved in the process of invasion and metastasis of esophageal carcinoma. Combined detection of AEG-1 and E-ca is an important factor in judging metastasis potential and prognosis of esophageal cancer patients. 2. AEG-1 may mediate the down-regulation of E-ca expression in esophageal carcinoma.
【学位授予单位】：新乡医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.1

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