食管鳞癌中差异表达的lncRNA的筛选及分析

发布时间：2018-03-23 21:12

  本文选题：长链非编码RNA　切入点：食管鳞癌　出处：《现代肿瘤医学》2017年15期

【摘要】：目的:筛查及分析食管鳞癌中长链非编码RNA(lncRNA)及mRNA的差异表达谱,以及这些差异lncRNAs的功能、互作分子。方法:通过对3对食管鳞癌/癌旁组织的基因芯片筛选及生物信息学分析,预测这些差异表达的lncRNAs潜在靶点、通路,及其与临近编码基因、转录因子的相关性。结果:按FC值≥2.0且P值≤0.05的标准,共筛选得到癌及癌旁组织差异表达的lncRNA 182个,mRNA 108个,GO聚类和KEGG分析发现差异lncRNAs潜在靶标基因与细胞周期调控、细胞分化、细胞外基质等活性密切相关,进一步生物分析得到lncRNA NONHSAT015779、NONHSAT08197、NONHSAT112918和NONHSAT115190等17个lncRNAs可能与食管鳞癌发生发展密切相关。结论:与癌旁组织相比,lncRNAs在食管鳞癌组织中显示不同的表达模式,这些差异表达的lncRNAs可能在食管鳞癌发生和发展中发挥重要作用。
[Abstract]:Objective: to screen and analyze the differential expression profiles of long chain noncoding RNAs and mRNA in esophageal squamous cell carcinoma, and the function and interaction of these lncRNAs. Methods: three pairs of esophageal squamous cell carcinoma / paracancerous tissues were screened by microarray and analyzed by bioinformatics. Potential targets, pathways, and their correlation with adjacent coding genes and transcription factors were predicted for these differentially expressed lncRNAs. Results: according to the criteria of FC 鈮，

本文编号：1655174