MicroRNA-3198，microRNA-8084，microRNA-7515和microRNA-3613-3P在卵

发布时间：2018-03-24 03:14

  本文选题：卵巢肿瘤　切入点：微小核糖核酸　出处：《河北医科大学》2017年硕士论文

【摘要】：目的:卵巢上皮性肿瘤(Epithelial ovarian tumor)是女性常见生殖器肿瘤之一,是影响女性生命及生活质量的疾病之一,包括良性、交界性和恶性。而卵巢上皮性恶性肿瘤(Epithelial ovarian cancer,EOC)是常见妇科恶性肿瘤之一,其病死率居女性肿瘤中的第5位。微小核糖核酸(Micro RNA,miRNA)是一类长约19~24nt的非编码小RNA,在转录后水平调节基因的表达。在很多生物学过程,包括肿瘤发生发展、侵袭与转移、耐药和复发等过程中均可发现miRNA的表达。本实验检测初治卵巢上皮性癌原发灶、初治卵巢上皮性癌转移灶、复发卵巢上皮性癌和卵巢上皮性良性肿瘤组织中,miRNA-3198、miRNA-8084、miRNA-7515和miRNA-3613-3P四种miRNA的表达情况,比较各组表达差异,并探讨其可能的机制,以期发现miRNA在卵巢上皮性癌的发生、侵袭与转移及复发中的作用,为卵巢上皮性癌的诊治提供新的方向。方法:1无菌条件下收集在白求恩国际和平医院从2010年10月至2016年11月手术的临床组织标本,共60例,包括初治卵巢上皮性癌原发灶组织(A组)15例、初治卵巢上皮性癌转移灶组织(B组)15例、复发卵巢上皮性癌组织(C组)16例和卵巢上皮性良性肿瘤组织(D组)14例。所有组织标本置于-80℃冰箱冷藏。初治卵巢上皮性癌所有患者手术前均未行任何相关手术及辅助治疗。复发卵巢上皮性癌所有患者满足二次减灭术的适应症:距离最后一次化疗结束后复发间隔时间6~12个月;肿瘤病灶孤立且可完整切除;无腹水。所有标本均有术后病理确诊。2实时荧光定量聚合酶链式反应(Quantitative Real-time polymerase chain reaction,q RT-PCR)检测并用统计学方法分析结果。结果:1 miRNA-3198在A、B、C和D四组中表达情况的比较:A、B、C和D四组miRNA-3198的表达水平不全相同,具有统计学差异(χ2=20.32,P0.001)。进一步进行两两比较,结果是:B组miRNA-3198的表达水平与其余三组均不相同,均具有统计学差异(P0.05),B组是四组中miRNA-3198表达水平最低的一组。另外,A组低于D组,具有统计学差异(P0.05);C组低于D组,具有统计学差异(P0.05)。D组是四组中miRNA-3198表达水平最高的一组。而A组与C组的表达水平无统计学差异(P0.05)。2 miRNA-7515在A、B、C和D四组中表达情况的比较:A、B、C和D四组miRNA-7515的表达水平不全相同,具有统计学差异(χ2=19.23,P0.001)。进一步进行两两比较,结果是:A、B、C三组miRNA-7515的表达水平与D组均不相同,均具有统计学差异(P0.05),D组是四组中miRNA-7515表达水平最高的一组,而A、B、C三组miRNA-7515的表达水平无统计学差异(P0.05)。3 miRNA-8084在A、B、C和D四组中表达情况的比较:A、B、C和D四组miRNA-8084的表达水平不全相同,具有统计学差异(χ2=18.70,P0.001)。进一步进行两两比较,结果是:A、B、C三组miRNA-8084的表达水平与D组均不相同,均具有统计学差异(P0.05),D组是四组中miRNA-8084表达水平最高的一组,而A、B、C三组miRNA-8084的表达水平无统计学差异(P0.05)。4 miRNA-3613-3P在A、B、C和D四组中表达情况的比较:A、B、C和D四组miRNA-3613-3P的表达水平不全相同,具有统计学差异(χ2=17.81,P0.001)。进一步进行两两比较,结果是:B组和C组中miRNA-3613-3P的表达水平均低于A组,具有统计学差异(P0.05)。B组和C组miRNA-3613-3P的表达水平均低于D组,具有统计学差异(P0.05)。而A组和D组miRNA-3613-3P的表达水平无统计学差异(P0.05),B组和C组miRNA-3613-3P的表达水平无统计学差异(P0.05)。结论:1 miRNA-3198在初治卵巢上皮性癌原发灶、初治卵巢上皮性癌转移灶和复发卵巢上皮性癌组织中均为低表达,在初治卵巢上皮性癌转移灶组织中的表达水平最低,可能参与卵巢癌的发生发展、转移及复发。2 miRNA-7515在初治卵巢上皮性癌原发灶、初治卵巢上皮性癌转移灶和复发卵巢上皮性癌组织中均为低表达,可能参与卵巢癌的发生。3 miRNA-8084在初治卵巢上皮性癌原发灶、初治卵巢上皮性癌转移灶和复发卵巢上皮性癌组织中均为低表达,可能参与卵巢癌的发生。4 miRNA-3613-3P在初治卵巢上皮性癌转移灶和复发卵巢上皮性癌组织中均为低表达,可能参与卵巢癌的转移与复发。
[Abstract]:Objective: ovarian epithelial tumor (Epithelial ovarian tumor) is one of the common female genital tumors, is one of the female, and the quality of life of diseases including benign, borderline and malignant and malignant ovarian epithelial tumor (Epithelial ovarian, cancer, EOC) is one of the most common gynecologic malignant tumor, the mortality rate among women in tumor fifth. MicroRNAs (Micro RNA miRNA) is a class of about 19~24nt non small RNA encoding, regulate gene expression at the post transcriptional level. In many biological processes, including tumorigenesis, invasion and metastasis, the expression of miRNA can be found in the process of drug resistance and relapse. The early detection treatment of ovarian carcinoma, metastasis of early treatment of ovarian cancer, recurrent epithelial ovarian cancer and benign ovarian epithelial tumor tissues, miRNA-3198, miRNA-8084, miRNA-7515 and miRNA-3613-3P four M The expression of iRNA, compare the expression differences, and to explore its possible mechanism, in order to find the miRNA in epithelial ovarian cancer, invasion and metastasis and recurrence of the role, to provide a new direction for the diagnosis and treatment of ovarian cancer. Methods: in Heping Hospital from October 2010 to November 2016 Bethune International clinical surgery 1 specimens were collected under sterile conditions, a total of 60 cases, including primary tumor tissue of untreated epithelial ovarian cancer (A group) 15 cases, metastatic tissue of untreated epithelial ovarian cancer (B group) 15 cases, the recurrence of epithelial ovarian cancer (C group) and 16 cases of benign ovarian epithelial tumors (D group) 14 cases. All tissue specimens were placed in the refrigerator. C -80 initial treatment of ovarian cancer in all patients before surgery had no surgery and adjuvant treatment of recurrent epithelial ovarian cancer. All patients meet the two cytoreductive surgery indications: the last After the end of chemotherapy and recurrence interval of 6~12 months; tumor lesions were isolated and complete resection; no ascites. All specimens had postoperative real-time fluorescence quantitative polymerase chain reaction (Quantitative Real-time.2 and pathological diagnosis of polymerase chain reaction, Q RT-PCR) detection and statistical analysis results. Results: 1 miRNA-3198 in A, B, expression the comparison between the four groups in C and D: A, B, C and D expression level of miRNA-3198 in the four groups are not all the same, with statistical difference (2=20.32, P0.001). A further 22 comparison, the result is: the expression level of B in group miRNA-3198 and the other three groups are not the same, the differences were all statistically significant (P0.05), B group is the lowest level of the expression of miRNA-3198 in the four groups of a group. In addition, the A group was lower than that of D group, with statistical difference (P0.05); C group than in D group, with statistical difference (P0.05) of.D group is the four group the expression of miRNA-3198 in the highest level of a 缁，

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