肺癌患者LUNXmRNA表达的意义及其与中医证型的相关性研究

发布时间：2018-03-24 07:32

本文选题：肺特异性蛋白　切入点：肺泡灌洗液　出处：《安徽中医药大学》2015年硕士论文

【摘要】：目的探讨将肺泡灌洗液中肺特异性蛋白LUNXm RNA用于辅助肺癌诊断及辅助中医辨证的可行性;评价外周血肺特异性蛋白LUNXm RNA作为肺癌微转移的特异性标志物的价值及其与中医证型的相关性方法对接受纤维支气管镜的病例采集支气管肺泡灌洗液标本,对符合条件的病例采集外周血标本,提取标本中的总RNA,加入含目的基因引物的反转录系统反转录生成c DNA,运用RT-PCR方法对肺泡灌洗液和外周血的肺特异性蛋白LUNXm RNA进行检测,比较肺泡灌洗液的肺特异性蛋白LUNXm RNA在肺癌患者与非肺癌患者之间的表达差异,并分析检测值在肺癌不同证型之间的分布特点;分析外周血的阳性检出率与肺癌TNM分期的关系及其在肺癌中医证型间的分布规律。结果肺癌组和结核组患者肺泡灌洗液中肺特异性蛋白LUNXm RNA的表达水平存在统计学差异(结核组检测值高于肺癌组),各证型间肺泡灌洗液LUNXm RNA表达水平有显著差异,其中痰湿内盛证、阴虚毒热证表达含量最高(两者之间差异无统计学意义P0.05),气滞血瘀证次之,气阴两虚证最低;在肺癌III期、IV期及肺癌阴虚毒热证、气阴两虚证中外周血LUNXm RNA有较高的阳性检出率,且肺癌各证型间外周血LUNXm RNA阳性检出率差异具有统计学意义(P均0.05)。结论肺泡灌洗液LUNXm RNA辅助肺癌诊断不具备特异性,但与肺癌中医辨证分型有关,多与痰、热、毒等病理因素有关;外周血LUNXm RNA可用于检测肺癌微转移,但灵敏度欠佳,与肺癌中医证型有关,在阴虚毒热、气阴两虚等虚证证型中检出率高。
[Abstract]:Objective to explore the feasibility of using lung specific protein (LUNXm RNA) in alveolar lavage fluid to assist lung cancer diagnosis and TCM syndrome differentiation. To evaluate the value of peripheral blood lung specific protein LUNXm RNA as a specific marker for micrometastasis of lung cancer and its correlation with TCM syndromes the bronchoalveolar lavage fluid (BALF) samples were collected from patients receiving fiberoptic bronchoscopy. Peripheral blood samples were collected from eligible patients. Total RNAs were extracted from the samples. Reverse transcription system containing target gene primers was added to reverse transcription system to produce c DNA. the lung specific protein LUNXm RNA in alveolar lavage fluid and peripheral blood was detected by RT-PCR method. To compare the expression of lung specific protein LUNXm RNA in alveolar lavage fluid between lung cancer patients and non-lung cancer patients, and to analyze the distribution characteristics of the detection values among different types of lung cancer. To analyze the relationship between the positive detection rate of peripheral blood and TNM stage of lung cancer and its distribution in TCM syndrome of lung cancer. Results the expression level of lung specific protein LUNXm RNA in alveolar lavage fluid of lung cancer patients and tuberculosis patients was statistically analyzed. There were significant differences in the expression of LUNXm RNA in alveolar lavage fluid between different syndromes, the detection value in tuberculosis group was higher than that in lung cancer group. The expression of phlegm and dampness and yin deficiency and toxin heat syndrome were the highest (there was no significant difference between the two groups (P 0.05), Qi stagnation and blood stasis syndrome was the second, qi and yin deficiency syndrome was the lowest, in III stage IV of lung cancer and yin deficiency heat syndrome of lung cancer, there was no significant difference between them. The positive rate of LUNXm RNA in peripheral blood of Qi-Yin deficiency syndrome was higher, and the positive rate of LUNXm RNA in peripheral blood was significantly different among different types of lung cancer (P < 0.05). Conclusion LUNXm RNA in alveolar lavage fluid is not specific in the diagnosis of lung cancer. However, LUNXm RNA in peripheral blood can be used to detect micrometastasis of lung cancer, but its sensitivity is not good, and it is related to TCM syndrome type of lung cancer. The positive rate of deficiency syndrome such as deficiency of qi and yin is high.
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2

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