食管鳞状细胞癌中受体相互作用蛋白激酶1的表达及临床意义

发布时间：2018-03-24 08:02

本文选题：食管肿瘤　切入点：鳞状细胞　出处：《安徽医科大学》2016年硕士论文

【摘要】：目的本文旨在利用Western blot法和免疫组化等技术研究受体相互作用蛋白激酶1(RIP1)蛋白在食管鳞状细胞癌中的表达水平,并结合临床病理资料研究其在食管鳞癌中的表达水平的相关临床意义。方法应用Western blot法和免疫组化法检测,分析100例食管鳞癌患者的癌组织、相应癌旁组织(距病灶边缘2cm以内)和对应正常黏膜组织(距病灶边缘5 cm外)中RIP1的表达水平。以正常食管粘膜组织作为阴性对照,研究RIP1在食管癌组织及对应病例癌旁组织中的差异表达,应用统计学进一步分析RIP1表达水平与病患年龄、性别、临床病理分期等相关关系。结果71%食管鳞状上皮恶性肿瘤组织中RIP1蛋白的表达水平高于对应癌旁组织和正常黏膜组织,20%食管鳞状上皮恶性肿瘤旁组织中RIP1蛋白的表达水平高于对应食管鳞癌组织和正常食管黏膜中蛋白的表达水平,差异有统计学意义(P0.05)。食管鳞癌组织中高表达的RIP1蛋白水平与其TNM分期、病理分级、是否存在周围淋巴结转移(P0.05),与性别、年龄无关。癌旁组织中高表达的RIP1蛋白水平与肿瘤浸润深度、TNM分期有关(P0.05),与性别、年龄、是否存在周围淋巴结转移及病理分级无关。结论RIP1蛋白在食管鳞癌中的高表达可能与肿瘤的病理分级、TNM分期有关,对于食管鳞癌的发生发展有一定的预测作用。
[Abstract]:Objective to investigate the expression of receptor interacting protein kinase 1 (RIP1) protein in esophageal squamous cell carcinoma by Western blot and immunohistochemistry. The clinical significance of the expression level in esophageal squamous cell carcinoma was studied with clinicopathological data. Methods Western blot method and immunohistochemical method were used to analyze the cancer tissues of 100 patients with esophageal squamous cell carcinoma. The expression of RIP1 in the corresponding paracancerous tissues (within 2cm) and the corresponding normal mucosa (5 cm away from the edge of the lesion) was detected. The normal esophageal mucosa was used as the negative control. To study the differential expression of RIP1 in esophageal carcinoma tissues and adjacent tissues of corresponding cases, and to further analyze the expression level of RIP1 with age and sex by statistical analysis. Results the expression level of RIP1 protein in 71% malignant esophageal squamous carcinoma tissues was higher than that in the corresponding paracancerous tissues and normal mucosa tissues of 20% esophageal squamous carcinoma tissues. The expression level of protein in esophageal squamous cell carcinoma tissues and normal esophageal mucosa was higher than that in corresponding esophageal squamous cell carcinoma tissues. The high expression of RIP1 protein in esophageal squamous cell carcinoma was correlated with its TNM stage, pathological grade, whether there were peripheral lymph node metastasis (P0.05), and sex. The high expression of RIP1 protein in paracancerous tissues was related to the stage of tumor invasion depth (P0.05), sex, age, and age. Conclusion the high expression of RIP1 protein in esophageal squamous cell carcinoma may be related to the pathological grade and staging of the tumor, which may predict the occurrence and development of esophageal squamous cell carcinoma.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.1

【参考文献】