senex基因在急性髓系白血病中的表达及意义

发布时间：2018-03-24 17:16

本文选题：急性髓系白血病　切入点：senex　出处：《安徽医科大学学报》2017年03期

【摘要】：目的探讨senex基因在急性髓系白血病(AML)中的表达水平,并探讨其在AML发生发展中的意义。方法应用实时荧光定量PCR检测senex基因在21例初诊AML患者(初诊组)、27例骨髓完全缓解的AML患者(缓解组)、19例骨髓复发的AML患者(复发组)以及12例对照者(对照组)的骨髓单个核细胞中的表达情况,比较4组之间的基因表达水平差异,并分析senex基因表达水平与临床特征和疗效的关联性。结果初诊组senex基因表达水平高于缓解组(4.06±2.72 vs 2.52±1.83,P=0.04)和对照组(4.06±2.72 vs 1.22±0.48,P=0.001),复发组senex基因表达水平高于对照组(3.27±2.54 vs 1.22±0.48,P=0.017),且各亚型之间以AML-M3的senex基因表达水平最高。AML患者senex基因mRNA表达水平与骨髓原始细胞比例存在相关性(rs=0.557,P=0.000)。此外分析AML初诊患者的临床特征显示1次化疗缓解的患者(n=14)的senex基因表达显著低于1次化疗未缓解的患者(n=7)(1.77±1.47 vs 4.85±2.20,P=0.003),并且1次化疗未缓解的患者预后均较差。结论 senex基因的异常表达可能与AML的发生发展以及预后密切相关。
[Abstract]:Objective to investigate the expression of senex gene in acute myeloid leukemia (AML). Methods Real-time quantitative PCR was used to detect the senex gene in 21 patients with newly diagnosed AML (27 patients with complete remission of bone marrow in AML patients) and 19 patients with AML with bone marrow recurrence in remission group (n = 19). The expression of bone marrow mononuclear cells (BMC) in the recurrent group (n = 12) and control group (n = 12). The differences of gene expression levels among the four groups were compared. Results the expression level of senex gene in the newly diagnosed group was higher than that in the remission group (4.06 卤2.72 vs 2.52 卤1.83) and the control group (4.06 卤2.72 vs 1.22 卤0.48Pn.001). The expression level of senex gene in the recurrent group was higher than that in the control group (3.27 卤2.54 vs 1.22 卤0.48P0. 017), and the expression level of senex gene in the recurrent group was higher than that in the control group (3. 27 卤2. 54 vs 1. 22 卤0. 48). MRNA expression of senex gene in patients with AML-M3 was the highest among subtypes. There was a correlation between mRNA expression of senex gene and the ratio of bone marrow progenitor cells. In addition, the senex of AML patients with primary remission was analyzed by analyzing the clinical characteristics of AML patients. The expression of senex gene was significantly lower than that of the patients without remission after one chemotherapy (1.77 卤1.47 vs 4.85 卤2.20P0. 003), and the prognosis of the patients without remission was poor. Conclusion the abnormal expression of senex gene may be closely related to the occurrence, development and prognosis of AML.
【作者单位】：安徽医科大学第二附属医院血液科安徽医科大学血液病研究中心;
【基金】：国家自然科学基金(编号:81670179、81401293)
【分类号】：R733.71

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