高良姜素诱导人乳头瘤病毒阳性的宫颈癌细胞凋亡实验研究

发布时间：2018-03-25 09:45

本文选题：高良姜素　切入点：宫颈癌　出处：《中草药》2017年05期

【摘要】：目的研究高良姜素对人乳头瘤病毒(HPV)阳性人宫颈癌细胞的诱导凋亡作用及其机制。方法选择2株HPV阳性人宫颈癌细胞(Si Ha、He La)和1株HPV阴性人宫颈癌细胞(C-33-A),用不同浓度的高良姜素(20、40、80μmol/L)分别处理24、48、72 h后,采用噻唑蓝(MTT)法测定高良姜素对3株宫颈癌细胞增殖的影响;不同浓度高良姜素处理宫颈癌细胞48 h后,分别采用流式细胞仪检测细胞凋亡和细胞周期;Western blotting法检测Bcl-2家族蛋白表达情况。结果高良姜素可以剂量和时间依赖性地降低HPV阳性宫颈癌细胞Si Ha和He La的活力;而对于HPV阴性的宫颈癌细胞C-33-A活力无明显影响;细胞周期检测显示高良姜素可以使Si Ha和He La细胞的生长阻滞在G1或G2/M期;流式细胞仪分析发现40μmol/L高良姜素作用于Si Ha和He La细胞48 h后,细胞出现明显凋亡,而C-33-A细胞仅有少量凋亡;Western blotting检测发现40μmol/L高良姜素处理后,HPV阳性宫颈癌细胞中促凋亡蛋白Bad、Bid、Bax表达量升高,而抗凋亡蛋白Bcl-2、Bcl-w表达量降低。结论高良姜素能抑制HPV阳性宫颈癌细胞的增殖并诱导其凋亡,其作用机制可能与下调Bcl-2、Bcl-w基因的表达及上调Bad、Bid、Bax基因的表达有关。
[Abstract]:Objective to study the apoptosis of human cervical cancer cells positive for human papillomavirus (HPV) and its mechanism. Methods two HPV positive human cervical cancer cell lines, si-Haha-Lahe) and one HPV negative human cervical cancer cell line, C-33-An, were selected and compared with each other. The concentration of gingerin 20, 40, 80 渭 mol / L, respectively, was treated for 24, 48, 872 hours, The effects of alpinol on the proliferation of three cervical cancer cell lines were determined by thiazolyl methacrylate (MTT) method, and the cells were treated with different concentrations of alpinol for 48 h. Apoptosis was detected by flow cytometry and the expression of Bcl-2 family protein was detected by Western blotting. Results Gingerin could decrease the activity of SIHA and He-La in a dose-and time-dependent manner. Cell cycle analysis showed that gingerin could block the growth of SIHA and He-La cells in G _ 1 or G _ 2 / M phase, but had no effect on C-33-A activity of cervical cancer cells with negative HPV. Flow cytometry analysis showed that 40 渭 mol/L gingerin induced apoptosis in SIHA and He-La cells for 48 h. However, only a small amount of apoptosis was detected by Western blotting assay in C-33-A cells. It was found that the expression of apoptotic protein Badbidophane Bax in HPV-positive cervical cancer cells was increased after treatment with 40 渭 mol/L gingerin. Conclusion Gingerin can inhibit the proliferation and induce apoptosis of HPV positive cervical cancer cells. The mechanism may be related to the down-regulation of Bcl-2P Bcl-w gene expression and the up-regulation of Badbidophane Bax gene expression.
【作者单位】：郑州大学附属郑州中心医院;
【分类号】：R737.33

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