胃癌外周血微小RNA-23a表达及其与临床特征相关性分析

发布时间：2018-03-25 12:51

本文选题：微小RNA-23a　切入点：外周血　出处：《浙江大学》2015年硕士论文

【摘要】：背景及目的胃癌是危害我国人民身体健康的重大疾病。胃癌防控的关键在于早期诊断和早期治疗。目前胃癌的筛查和早期诊断依赖胃镜检查,费用高,患者接受度低。缺乏用于胃癌诊断外周血分子标志物。本实验室前期关于外周血miRNA高通量测序的研究发现多个在胃癌患者中和健康人群中差异表达的miRNA,包其中括miR-23a.本研究旨在不同人群中收集外周血,分析其miR-23a表达量,以期发现其表达量与胃癌的关系以及与临床和病理特征的相关性,进而评估其是否有成为胃癌外周血检测分子标志物的可能性。方法在通过医院伦理委员会批准以及患者知情同意的情况下收集邵逸夫医院就诊的患者外周血及临床资料,采用高速离心的方式获取血清,后通过实时荧光定量逆转录PCR的方法检测血清中miR-23a表达量,结合检测结果及临床资料,采用独立样本的t检验、卡方检验及spearman相关性分析其与胃癌的相关性。结果 1)胃癌组外周血miR-23a表达量较健康对照组显著升高,差异具有统计学意义。 2)以外周血miR-23a表达量ACT值12.335为阳性判断标准时,对于胃癌的判断灵敏度为63%,特异度为60%。胃癌组中阳性患者淋巴结转移率较高,与阴性者比较有统计学意义。 3)在胃癌患者中外周血1niR-23a表达量与印戒细胞癌呈负相关,及肿瘤的分化程度呈正相关。结论外周血miR-23a作为胃癌诊断的外周血分子标志物可能有有助于胃癌患者预后的判断。
[Abstract]:Background and purpose. Gastric cancer is a major disease that endangers the health of our people. The key to the prevention and control of gastric cancer lies in early diagnosis and treatment. At present, the screening and early diagnosis of gastric cancer depends on gastroscopy and the cost is high. Low acceptance of patients. Lack of molecular markers for the diagnosis of gastric cancer peripheral blood. Our previous study of peripheral blood miRNA high-throughput sequencing found a number of differentially expressed miRNAs in patients with gastric cancer and in healthy people, including. The aim of this study was to collect peripheral blood from different populations. The expression of miR-23a was analyzed in order to find out the relationship between the expression of miR-23a and gastric cancer and its correlation with clinical and pathological features, and to evaluate the possibility of its expression as a molecular marker in peripheral blood detection of gastric cancer. Method. With the approval of the Hospital Ethics Committee and the informed consent of the patients, the peripheral blood and clinical data of the patients treated by Shaw Hospital were collected, and the serum was obtained by high-speed centrifugation. The expression of miR-23a in serum was detected by real-time fluorescence quantitative reverse transcription (PCR). The correlation between miR-23a and gastric cancer was analyzed by t-test of independent samples, chi-square test and spearman correlation analysis. Results. 1) the expression of miR-23a in peripheral blood of gastric cancer group was significantly higher than that of healthy control group. 2) when the ACT value of miR-23a expression in peripheral blood was taken as the positive criterion, the sensitivity and specificity for gastric cancer were 63 and 600.The lymph node metastasis rate of the positive patients was higher than that of the negative patients. 3) in gastric cancer patients, the expression of 1niR-23a in peripheral blood was negatively correlated with signet ring cell carcinoma and positively correlated with the degree of tumor differentiation. Conclusion. Peripheral blood miR-23a as a molecular marker in the diagnosis of gastric cancer may be helpful to the prognosis of gastric cancer patients.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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