过表达Tg737基因对肝癌细胞周期和凋亡的影响

发布时间：2018-03-25 14:29

本文选题：癌　切入点：肝细胞　出处：《中国普通外科杂志》2017年03期

【摘要】：目的:探讨Tg737基因过表达对人肝癌细胞株细胞周期和凋亡的影响及可能的机制。方法:将肝癌HepG2和MHCC97-H细胞分别用脂质体法转染pcDNA3.1-Tg737质粒(Tg737过表达组)或pcDNA3.1空载体(空载体组),或单纯脂质体孵育(脂质体组),以各自未处理的细胞为空白对照。48h后分别用流式细胞仪检测细胞周期与凋亡,Hoechst33342染料法检测细胞核形态,Westernblot法检测cyclinA、Bax、Bcl-2表达。结果:与各自的空白对照组比较,Tg737过表达组HepG2和MHCC97-H细胞的S期细胞数量与细胞凋亡率均明显增加(均P0.05),且细胞核均出现明显凋亡形态学改变,同时伴随cyclinA、Bax的表达上调和Bcl-2的下调(均P0.05);空载体组、脂质体组HepG2和MHCC97-H细胞镜下未见明显的凋亡形态学变化,且上述指标差异均无统计学意义(均P0.05)。结论:Tg737基因过表达可以抑制肝癌细胞周期进程、促进其凋亡,机制可能与Tg737参与调节cyclinA、Bax、Bcl-2有关的信号通路有关。
[Abstract]:Objective: to investigate the effect of overexpression of Tg737 gene on cell cycle and apoptosis of human hepatoma cell line and its possible mechanism. Methods: HepG2 and MHCC97-H cells were transfected with pcDNA3.1-Tg737 plasmid Tg737 or pcDNA3.1 empty vector by liposome respectively. Empty carrier group or pure liposome incubated (liposome group). 48 hours later, flow cytometry was used to detect cell cycle and apoptosis. Hoechst33342 dye method was used to detect nuclear morphology and Western blot. Results: compared with the control group, the S phase cell number and apoptosis rate of HepG2 and MHCC97-H cells in Tg737 overexpression group were significantly increased (P 0.05), and the morphological changes of apoptosis were observed in the nuclei. At the same time, the expression of cyclin Agna Bax was up-regulated and the Bcl-2 was down-regulated (all P 0.05), but there were no obvious morphological changes of apoptosis in HepG2 and MHCC97-H cells in empty vector group, liposome group and liposome group under microscope. Conclusion the overexpression of Tg737 gene can inhibit the cell cycle progression and promote the apoptosis of hepatoma cells, and the mechanism may be related to the regulation of the signal pathway related to cyclin Agna BaxC-2 by Tg737.
【作者单位】：第三军医大学新桥医院肝胆外科;
【基金】：国家自然科学基金资助项目(81402032)
【分类号】：R735.7

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