宫颈癌组织AKT和P-AKT表达与LMVD相关性及其对新辅助化疗效果预测意义

发布时间：2018-03-25 15:17

本文选题：宫颈癌　切入点：新辅助化疗　出处：《中华肿瘤防治杂志》2017年13期

【摘要】：目的宫颈癌新辅助化疗(neoadjuvant chemotherapy,NACT)的疗效一直存在争议,PI3K/AKT通路在肿瘤细胞增殖、迁移、侵袭及抗凋亡都起到举足轻重的作用。本研究探讨NACT对宫颈鳞状细胞癌AKT通路中AKT及P-AKT的表达对肿瘤微淋巴管生成的影响,并分析其变化与NACT临床疗效的关系。方法采用免疫组化SP法分别检测2011-03-01-2016-03-31滨州医学院附属医院收治的47例ⅠB2~ⅡB期宫颈鳞癌患者行NACT前后AKT和P-AKT的表达情况及肿瘤微淋巴管密度(lymphatic microvessel density,LMVD)的变化。结果免疫组化检测结果显示,NACT前AK和P-AKT的阳性率分别为93.6%和72.3%,化疗后阳性率分别为44.7%和36.2%,NACT前后比较,AKT和P-AKT表达差异有统计学意义,P0.001。化疗前宫颈鳞癌组织中LMVD计数为20.87±3.31,化疗后宫颈鳞癌组织中LMVD计数为16.47±3.44,化疗后宫颈鳞癌组织中LMVD明显降低,t=6.32,P0.001。化疗前宫颈鳞癌AKT阳性的LMVD为21.14±3.19,高于阴性组(17.00±3.00)。Spearman相关分析显示,化疗前后LMVD的表达(r_s=0.39,P=0.007)与化疗前后P-AKT的表达(r_s=0.66,P0.001)均呈正相关。化疗后LMVD与化疗后AKT的表达呈正相关,r_s=0.58,P0.001。淋巴结转移与化疗前后LMVD均呈正相关(r_s=0.44,P0.001;r_s=0.36,P=0.013)。淋巴结转移的患者化疗前化疗后LMVD均高于淋巴结未转移者。结论化疗前P-AKT的表达及化疗后AKT和P-AKT的表达与肿瘤微淋巴管生成呈正相关,LMVD与淋巴结的转移呈正相关,化疗前P-AKT的表达有可能作为预测宫颈癌新辅助化疗疗效的指标。
[Abstract]:The purpose of neoadjuvant chemotherapy for cervical cancer (neoadjuvant, chemotherapy, NACT) the curative effect has been controversial, the PI3K/AKT pathway in tumor cell migration, proliferation, invasion and apoptosis have played a pivotal role. This study was to investigate the expression of NACT on AKT and P-AKT in cervical squamous cell carcinoma in AKT pathway on tumor lymphangiogenesis effect to analyze the relationship between NACT changes and clinical curative effect. Methods: SP immunohistochemical method was used to detect 2011-03-01-2016-03-31 in Affiliated Hospital of Binzhou Medical College were 47 cases of B2~ stage B cervical squamous cell carcinoma before and after NACT in patients with AKT and P-AKT expression and tumor lymphatic microvessel density (lymphatic microvessel, density, LMVD) changes. Results the results of immunohistochemistry the results showed that the positive rate of NACT AK and P-AKT were 93.6% and 72.3% after chemotherapy, the positive rates were 44.7% and 36.2%, NACT compared AKT and P-AKT table There are statistically significant differences of P0.001., LMVD before chemotherapy in cervical squamous cell carcinoma count was 20.87 + 3.31, 16.47 + 3.44 in the count of LMVD cervical squamous cell carcinoma LMVD cervical squamous cell carcinoma after chemotherapy, chemotherapy harem was significantly lower, t=6.32, P0.001. before chemotherapy of cervical squamous cell carcinoma with AKT positive LMVD was 21.14 + 3.19, higher than the negative group (17 + 3).Spearman correlation analysis showed that the expression of LMVD before and after chemotherapy (r_s=0.39, P=0.007) and the expression of P-AKT before and after chemotherapy (r_s=0.66, P0.001) were positively correlated. After chemotherapy, LMVD and AKT after chemotherapy of r_s= expression was 0.58, lymph node metastasis and chemotherapy before and after P0.001. LMVD were positively correlated (r_s=0.44, P0.001; r_s=0.36, P=0.013). Chemotherapy in patients with lymph node metastasis before LMVD after chemotherapy were higher than those without lymph node metastasis. Conclusion the expression of P-AKT after chemotherapy before chemotherapy and the expression of AKT and P-AKT with tumor lymphangiogenesis was positively related to LM The expression of VD is positively correlated with the metastasis of lymph nodes. The expression of P-AKT before chemotherapy may be an indicator of the efficacy of new adjuvant chemotherapy for cervical cancer.

【作者单位】：滨州医学院附属医院妇科;
【分类号】：R737.33

【相似文献】