信号传导及转录激活因子3基因在老年甲状腺癌组织中的表达及基因沉默对甲状腺癌细胞的影响

发布时间：2018-03-27 05:25

本文选题：信号传导及转录激活因子　切入点：甲状腺癌　出处：《中国老年学杂志》2017年24期

【摘要】：目的观察信号传导及转录激活因子(STAT)3基因在老年甲状腺癌组织中的表达及沉默STAT3基因对甲状腺癌细胞的影响。方法将FTC-133细胞分为siRNA STAT3组、阴性对照组和空白对照组,RT-PCR测定FTC-133细胞STAT3、基质金属蛋白酶(MMP)2、MMP9 mRNA的表达,Western印迹测定甲状腺癌FTC-133细胞STAT3蛋白的表达,MTT法测定甲状腺癌FTC-133细胞的生长,Transwell法检测FTC-133细胞的迁移能力。结果老年甲状腺癌组织中STAT3阳性率高于癌旁组织(P0.05)。siRNA STAT3组FTC-133细胞中STAT3蛋白的表达量低于阴性对照组和空白对照组(P0.05)。siRNA STAT3组FTC-133细胞中STAT3、MMP2、MMP9 mRNA的表达量低于阴性对照组和空白对照组(P0.05)。siRNA STAT3组第1、2、3天的抑制率均明显高于阴性对照组(P0.05)。siRNA STAT3组FTC-133细胞中穿膜细胞数低于阴性对照组和空白对照组(P0.05)。结论老年甲状腺癌组织中STAT3呈高表达,沉默STAT3基因对甲状腺癌细胞株的增殖和侵袭能力有抑制作用。
[Abstract]:Objective to investigate the expression of signal transduction and transcriptional activator statin 3 gene in elderly thyroid carcinoma tissues and the effect of silencing STAT3 gene on thyroid cancer cells. Methods FTC-133 cells were divided into siRNA STAT3 group. The expression of STAT3 in FTC-133 cells was detected by RT-PCR and the expression of MMP9 mRNA in FTC-133 cells of thyroid carcinoma was detected by Western blot. The growth of FTC-133 cells in thyroid carcinoma was detected by transwell method. The expression of STAT3 protein in FTC-133 cells was detected by Western blotting. The growth of FTC-133 cells in thyroid carcinoma was detected by transwell method. Results the positive rate of STAT3 in the elderly thyroid carcinoma tissues was higher than that in the FTC-133 cells of the paracancerous tissues P0.05. siRNA STAT3 group. The expression of STAT3 protein in the FTC-133 cells was lower than that in the negative control group and the blank control group. The expression of STAT3 and MMP2MMP9 mRNA in FTC-133 cells was lower than that in the control group. The inhibition rate of FTC-133 cells in the control group and the blank control group was significantly higher than that in the negative control group (P0.05). SiRNA STAT3 group was significantly lower than that in the negative control group and the blank control group. Conclusion the expression of STAT3 in the elderly thyroid carcinoma tissues was higher than that in the negative control group and the blank control group. Silencing STAT3 gene can inhibit the proliferation and invasion of thyroid cancer cell line.
【作者单位】：河北医科大学附属唐山工人医院头颈外科;
【分类号】：R736.1

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