哈萨克食管鳞癌组织中HSP27，SM22，TAGLN2，P38MAPK的表达及临床意义分析

发布时间：2018-03-30 07:50

本文选题：食管鳞状细胞癌　切入点：热休克蛋白27　出处：《新疆医科大学》2016年硕士论文

【摘要】：目的:对课题组筛选出的食管鳞癌组织中差异蛋白HSP27,SM22,TAGLN2和P38MAPK进行验证,并结合临床病理参数筛选可能的分子标记物。方法:运用Western Blot对28对新鲜食管鳞癌组织中的HSP27,SM22,TAGLN2和P38MAPK表达情况进行分析;再运用免疫组织化学技术对162例石蜡包埋食管鳞癌组织及50例食管粘膜正常组织中上述蛋白表达情况进行分析;选取部分石蜡包埋组织病例资料进行随访;结合临床病理参数进行数据统计,运用ROC曲线评估各蛋白诊断价值,运用Cox回归模型分析与食管鳞癌预后相关因素。结果:(1)Western Blot发现HSP27,SM22,TAGLN2和P38MAPK在食管鳞状细胞癌肿瘤组织中的表达升高(P0.05);HSP27,SM22在食管鳞状细胞癌淋巴结转移组表达升高(P0.05)。(2)免疫组织化学发现上述蛋白在石蜡包埋癌组织中的表达阳性率高于正常粘膜组织(P0.001);临床病理参数分析发现HSP27,SM22,TAGLN2表达阳性与食管癌淋巴结转移存在相关性(P0.05);SM22,TAGLN2,P38MAPK表达阳性与食管癌浸润深度存在相关性(P0.05);计算ROC曲线下面积发现TAGLN2有较高诊断价值(AUC=0.902,P0.001),SM22,HSP27,P38MAPK有一定的诊断价值。(3)通过Cox回归模型生存分析发现淋巴结转移、浸润深度、HSP27表达与患者生存情况存在相关性,差异有统计学意义(P0.05),而其它因素尚不能认为与患者远期生存存在相关性(P0.05)。结论:HSP27,SM22,TAGLN2,P38MAPK是食管鳞状细胞癌发生发展过程中的重要变化分子,可能对食管鳞状细胞癌恶性程度判断有一定的指导意义;TAGLN2作为食管鳞状细胞癌诊断的分子标记物有较高诊断价值;HSP27与食管鳞癌患者预后存在相关性,可以作为预后评估的分子标志物。
[Abstract]:Objective: to verify the differential protein HSP27SM22SM22 TAGLN2 and P38MAPK in esophageal squamous cell carcinoma. Methods: Western Blot was used to analyze the expression of Western Blot in 28 pairs of fresh esophageal squamous cell carcinoma tissues. The expression of these proteins in 162 paraffin-embedded esophageal squamous cell carcinoma tissues and 50 normal esophageal mucosa tissues were analyzed by immunohistochemical technique, and some paraffin embedded tissues were selected for follow-up. The ROC curve was used to evaluate the diagnostic value of each protein. Cox regression model was used to analyze the prognostic factors of esophageal squamous cell carcinoma. Results the expression of HSP27 SM22, TAGLN2 and P38MAPK in esophageal squamous cell carcinoma tumor tissues was increased by Blot. The positive rate of the above protein expression in paraffin embedded carcinoma tissue was higher than that in the normal mucosa tissue P0.001.The clinicopathologic parameters analysis showed that the expression of HSP27 SMM22 / TAGLN2 was correlated with lymph node metastasis of esophageal carcinoma. The area under the ROC curve was found to be of high diagnostic value by calculating the area under the ROC curve. There was a certain diagnostic value of TAGLN2 in SM22HSP27P38MAPK). Lymph node metastasis was found by survival analysis of Cox regression model. The expression of HSP27 was correlated with the survival of the patients. The difference was statistically significant (P 0.05), but other factors could not be considered to be related to the long-term survival of the patients. Conclusion: the proportion of HSP27, SM22, TAGLN2 and P38 MAPK is an important variable molecule in the carcinogenesis and development of esophageal squamous cell carcinoma. TAGLN2 may be a molecular marker for the diagnosis of esophageal squamous cell carcinoma. HSP27 may be associated with the prognosis of esophageal squamous cell carcinoma. It can be used as a molecular marker for prognostic evaluation.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.1

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