人肝细胞癌细胞DNA甲基化谱的检测及分析

发布时间：2018-03-30 15:18

  本文选题：肝细胞癌　切入点：甲基化谱　出处：《中国医科大学学报》2017年12期

【摘要】：目的检测人肝细胞癌细胞的DNA甲基化谱,明确肝细胞癌细胞中差异甲基化位点和基因的表达分布情况,进一步探讨DNA异常甲基化与肝细胞癌发生发展的关系。方法使用DNA甲基化芯片(Infinium Human Methylation 450K Bead Chip)检测人肝细胞癌细胞Huh7和人永生化肝细胞L02的甲基化谱,并对检测结果进行生物学分析。结果共检测到差异性甲基化位点102 254个,差异性甲基化基因26 511个,甲基化相关信号通路43个,其中57.3%的高甲基化CpG位点和39.4%的低甲基化CpG位点的甲基化差异程度≥50%,筛选后确定了3 222个显著高甲基化基因和2 204个显著低甲基化基因。结论在Huh7和L02细胞中存在大量差异性甲基化CpG位点及基因,Huh7细胞中可检测到大量抑癌基因DNA的异常高甲基化,提示DNA异常甲基化与肝细胞癌的发生发展密切相关。
[Abstract]:Objective to detect the DNA methylation spectrum of human hepatocellular carcinoma cells and to determine the distribution of differentially methylated sites and genes in hepatocellular carcinoma cells. Methods DNA methylation microarray was used to detect the methylation spectrum of human hepatocellular carcinoma cell line Huh7 and human immortalized liver cell L02. The results showed that there were 102,254 differentially methylated sites, 26,511 differentially methylated genes and 43 methylation-related signaling pathways. Among them, 57.3% of the hypermethylated CpG sites and 39.4% of the hypomethylated CpG sites had significant methylation differences of 鈮，

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