miR-126-5p在胃癌组织中的表达及其意义

发布时间：2018-03-30 21:25

  本文选题：胃癌　切入点：miR-126-5p　出处：《兰州大学》2017年硕士论文

【摘要】：目的:研究miR-126-5p在胃癌组织及胃癌旁组织的表达,初步探讨miR-126-5p与胃癌发生、发展之间的关系。方法:利用基因芯片技术检测14例早癌患者的胃癌组织及其癌旁组织,进行miRNAs的表达谱情况分析;收集42例胃癌组织及其癌旁组织标本,利用实时荧光实时定量PCR(q RT-PCR)检测miR-126-5p的表达情况,依据实验结果来初步探讨在胃癌组织中miR-126-5p的表达情况与胃癌的产生、进展及胃癌患者的临床病理资料之间的相关性。结果:miR-126-5p在胃癌组织中的ΔCT值为5.440±1.650,在癌旁组织中ΔCT值为7.329±1.456,miR-126-5p在胃癌组织中表达上调的有34个,下调的有8个,miR-126-5p在胃癌组织中的表达是上调的,结果具有统计学意义。对癌组织及癌旁组织的ΔCT值做ROC曲线分析,得出ΔCT值ROC曲线下的面积是0.818,该指标对胃癌诊断有一定的准确性。所得到的最佳临界值为7.125。当ΔC T≤7.125时,其对胃癌诊断的灵敏性为88.10%。miR-126-5p相对表达量ΔCT与临床病理资料分析,结果示P值大于0.05,miR-126-5p在胃癌组织中的表达与临床病理资料无明显相关性。结论:miR-126-5p在胃癌组织中是高表达的,对胃癌具有一定的诊断价值。
[Abstract]:Objective: to study the expression of miR-126-5p in gastric carcinoma and its adjacent tissues, and to explore the relationship between miR-126-5p and carcinogenesis and development of gastric cancer. The expression profile of miRNAs was analyzed and the expression of miR-126-5p was detected by real-time quantitative PCR(q RT-PCR in 42 cases of gastric carcinoma and its adjacent tissues. According to the results of the experiment, the expression of miR-126-5p and the production of gastric cancer were preliminarily studied. Results the 螖 CT value of 1: miR-126-5p was 5.440 卤1.650 in gastric cancer tissue and 7.329 卤1.456miR-126-5p in paracancerous tissue. Eight down-regulated expression of miR-126-5p was up-regulated in gastric cancer tissues, and the results were statistically significant. The 螖 CT values of cancer tissues and adjacent tissues were analyzed by ROC curve. The area under the 螖 CT value ROC curve was 0.818, and this index had certain accuracy in the diagnosis of gastric cancer. The optimum critical value was 7.125. When 螖 C T 鈮，

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