基于NIR-SERS技术结合统计分析肺癌氧合血红蛋白

发布时间：2018-04-01 09:42

本文选题：肺癌　切入点：氧合血红蛋白　出处：《光谱学与光谱分析》2017年02期

【摘要】：基于新型、高效、生物兼容性近红外表面增强拉曼散射(NIR-SERS)基底,采用共焦光路设计的便携式拉曼光谱仪,对25例肺癌患者和25例健康人的氧合血红蛋白进行NIR-SERS光谱检测,再对两者NIR-SERS光谱的谱峰强度和谱峰位置进行分析研究。发现两者的NIR-SERS光谱的谱峰强度在一定区域存在较明显的差异性,在473,816和1 583cm~(-1)处健康人的光谱的谱峰强度比肺癌患者要大,健康人氧合血红蛋白平均NIR-SERS光谱在1 208和1 425cm~(-1)谱峰处时,肺癌患者分别蓝移到1 212和1 432cm~(-1),健康人氧合血红蛋白平均NIR-SERS光谱在1 583cm~(-1)的谱峰处,肺癌患者红移到1 579cm~(-1)。采用主成分(PCA)分析和独立变量T检验统计分析方法得出NIR-SERS光谱在主成分得分PC3,PC4和PC9处具有显著差异,并通过PCA得分二维、三维散点图可以将健康人和肺癌患者区分,诊断的特异性为88%,灵敏度为80%,总判别正确率为84%。通过谱峰归属分析,两者的NIR-SERS光谱存在显著差异与氧合血红蛋白吡咯环和乙烯基团(C_aC_m)的振动有关。该研究为肺癌的SERS光谱技术诊断提供了实验依据,NIR-SERS技术有望发展成为一种新型的临床辅助诊断工具。
[Abstract]:Based on a novel, efficient and biocompatible near-infrared surface-enhanced Raman scattering (NIR-SERS) substrate, the oxygenated hemoglobin in 25 patients with lung cancer and 25 healthy persons were detected by NIR-SERS spectroscopy using a portable Raman spectrometer designed by confocal light path. The peak intensity and position of NIR-SERS spectra were analyzed. It was found that the peak intensities of NIR-SERS spectra were significantly different in certain regions, and the peak intensities of healthy people at 473816 and 1583 cm ~ (-1) were higher than those of patients with lung cancer. At the peak of 1 208 and 1 425 cm ~ (-1) of oxygenated hemoglobin in healthy persons, the mean NIR-SERS spectra of lung cancer patients moved to 1 212 and 1 432 cm ~ (-1), respectively, and the mean NIR-SERS spectra of healthy people were at 1 583 cm ~ (-1). The red shift of lung cancer patients to 1579 cm ~ (-1) was obtained by using principal component analysis (PCA) and statistical analysis of independent variable T test (T-test). It was found that the NIR-SERS spectrum had significant difference between PC3mPC4 and PC9 in principal component score (PCA) and 2-D PCA score. Three-dimensional scatter plot can distinguish healthy people from patients with lung cancer. The specificity of diagnosis is 88. The sensitivity is 80. The total accuracy of discrimination is 84. The significant difference between the NIR-SERS spectra of the two groups is related to the vibration of the oxygenated hemoglobin pyrrole ring and the vinyl group Cad. This study provides an experimental basis for the diagnosis of lung cancer by SERS spectroscopy. The NIR-SERS technique is expected to develop into a new type of impending disease. Bed assistant diagnostic tool.
【作者单位】：遵义医学院基础医学院;楚雄师范学院光谱技术应用研究所;遵义医学院附属医院检验科;
【基金】：国家自然科学基金项目(13064001) 遵义医学院硕士启动基金项目(F-723)资助
【分类号】：R734.2;O657.37

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