瘦素对小鼠乳腺癌调节性T细胞的影响

发布时间：2018-04-02 23:03

本文选题：乳腺癌　切入点：瘦素　出处：《免疫学杂志》2017年08期

【摘要】：目的探讨瘦素对小鼠乳腺癌调节性T细胞的影响及生物学意义。方法培养4T1乳腺癌细胞株并接种于Balb/c小鼠建立乳腺癌模型,将成瘤小鼠随机分为对照组和实验组,分别用等量生理盐水(normal saline,NS)和瘦素(leptin)处理,监测小鼠肿瘤的生长;流式细胞技术检测各组Balb/c荷瘤小鼠外周血、淋巴结、脾脏中调节性T淋巴细胞的数量及比例变化;ELISA检测各组荷瘤小鼠血清TGF-β1、IL-10含量。结果与NS组比较,实验组肿瘤生长速度随瘦素质量浓度的增加而加快;外周血和脾脏中调节性T细胞比例随瘦素质量浓度的增加而升高(P0.05),淋巴结中调节性T细胞比例变化不明显(P0.05),血清中TGF-β1、IL-10的含量随瘦素质量浓度的增加而升高(P0.05)。结论瘦素通过上调乳腺肿瘤微环境中调节性T细胞的数量比例,分泌抑制因子,抑制机体肿瘤免疫,进而促进小鼠乳腺肿瘤的生长。
[Abstract]:Objective to investigate the effect of leptin on regulatory T cells of mouse breast cancer and its biological significance.Methods 4T1 breast cancer cell lines were cultured and inoculated into Balb/c mice to establish breast cancer model. The tumor-bearing mice were randomly divided into two groups: control group and experimental group. The mice were treated with the same amount of normal saline and leptin to monitor the growth of tumor.The quantity and proportion of regulatory T lymphocytes in peripheral blood, lymph nodes and spleen of Balb/c bearing mice were detected by flow cytometry.Results compared with NS group, the tumor growth rate of the experimental group was accelerated with the increase of leptin concentration.The proportion of regulatory T cells in peripheral blood and spleen increased with the increase of leptin concentration, while the proportion of regulatory T cells in lymph nodes did not change significantly (P 0.05). The content of TGF- 尾 1 and IL-10 in serum increased with the increase of leptin concentration.Conclusion leptin inhibits tumor immunity by upregulating the proportion of regulatory T cells in breast tumor microenvironment, secreting inhibitory factor, and then promoting the growth of breast tumor in mice.
【作者单位】：桂林医学院第二附属医院检验科;广西肿瘤免疫与微环境调控重点实验室;桂林医学院免疫学教研室;
【基金】：国家自然科学基金(81260317)
【分类号】：R737.9

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