齐墩果酸纳米粒与肝素钠纳米粒联合应用治疗肝癌的体内外研究

发布时间：2018-04-04 11:22

本文选题：齐墩果酸纳米粒　切入点：肝素钠纳米粒　出处：《西安交通大学学报(医学版)》2017年06期

【摘要】：目的考察自制齐墩果酸纳米粒(OPTN)与自制肝素钠纳米粒(HPTN)联用后对肝癌的体内外抑制作用,并通过对比单独用药的结果,研究两药联用是否具有协同作用。方法以香豆素6和曙红为标记物,考察人肝癌细胞HepG2和小鼠腹水型肝癌细胞HCa-F对两种纳米粒的摄取情况,利用水溶性四氮唑(WST-1)法和流式细胞仪测定两药合用对HepG2的协同抑制作用以及细胞凋亡情况。体内通过测定肿瘤抑制率和苏木精曙红染色(HE)法评估两药合用后的抗肿瘤效果。结果经过4h的孵育,在HepG2细胞和HCa-F细胞里均能观测到纳米粒发出的荧光,表明包载药物和荧光标记物的纳米粒能被两种细胞摄取;OPTN与HPTN联用能协同抑制肝癌细胞的活性;体内试验结果表明两药联用后能有效抑制肿瘤生长。结论 OPTN与HPTN联用后能产生协同作用,有效抑制肝癌细胞及肝癌实体瘤的生长,作用明显强于单独用药。
[Abstract]:Objective to investigate the inhibitory effect of oleanolic acid nanoparticles (OPTNN) and heparin sodium nanoparticles (HPTNN) on liver cancer in vitro and in vivo.Methods using coumarin 6 and eosin as markers, the uptake of two kinds of nanoparticles by human hepatoma cell line HepG2 and mouse ascites hepatoma cell line HCa-F was investigated.The synergistic inhibition and apoptosis of HepG2 were determined by water-soluble tetrazolium (WST-1) and flow cytometry.Tumor inhibition rate and hematoxylin eosin staining were used to evaluate the antitumor effect of the two drugs in vivo.Results the fluorescence of nanoparticles was observed in both HepG2 cells and HCa-F cells after incubated for 4 h, indicating that the nanoparticles containing drugs and fluorescent markers could be ingested by two kinds of cells and could inhibit the activity of hepatoma cells in combination with HPTN.In vivo test results showed that the combination of the two drugs can effectively inhibit tumor growth.Conclusion OPTN combined with HPTN can produce synergistic effect and inhibit the growth of hepatoma cells and solid tumor effectively, and the effect is obviously stronger than that of drug alone.
【作者单位】：大连医科大学;大连医科大学药学院;
【基金】：2016年度本科创新人才培养教学改革项目(No.2016111506010215,2016111507010321)~~
【分类号】：R735.7

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