CYFIP1过表达的鼻咽癌细胞株CNE2增殖、迁移能力观察

发布时间：2018-04-04 18:24

本文选题：鼻咽癌　切入点：鼻咽癌细胞　出处：《山东医药》2017年15期

【摘要】：目的观察胞质FMRP相互作用蛋白1(CYFIP1)过表达的鼻咽癌细胞株CNE2增殖、迁移能力变化。方法将含CYFIP1基因片段和空载片段的慢病毒转染鼻咽癌细胞株CNE2,分别作为实验组和空载组;以不进行转染操作的CNE2细胞为对照组。分别于培养24、48、72、96 h采用MTT实验观察各组细胞增殖情况(以OD值表示)。进行细胞克隆形成实验,待平板中出现肉眼可见克隆时,终止培养并计算克隆形成数。分别采用划痕实验和Transwell迁移实验观察细胞迁移能力,测量细胞迁移距离,记录穿膜细胞数。结果三组不同培养时间OD值相比,P均0.05。实验组、空载组和对照组克隆形成数分别为(414±29)、(426±30)、(381±59)个,三组相比,P均0.05。实验组、空载组、对照组细胞相对迁移距离分别为(6.71±0.10)、(9.82±0.12)、(9.98±0.10)mm,实验组细胞迁移距离小于空载组和对照组(P均0.05)。实验组、空载组、对照组穿膜细胞数分别为(37.33±1.53)、(62.67±5.03)、(62.13±4.36)个,实验组穿膜细胞数少于空载组和对照组(P均0.05)。结论 CYFIP1过表达的鼻咽癌细胞株CNE2迁移能力受到抑制。CYFIP1表达异常可能与鼻咽癌的浸润、发展有关。
[Abstract]:Objective To observe the effect of cytoplasmic FMRP interacting protein 1 (CYFIP1) expression in nasopharyngeal carcinoma cell line CNE2 proliferation, migration methods containing CYFIP1 gene fragment and the fragment of the empty lentivirus transfected nasopharyngeal carcinoma cell line CNE2, respectively as the experimental group and the group with no no-load; transfection operation of CNE2 cells as the control group. H were cultured 24,48,72,96 MTT to observe the situation of cell proliferation in each group (odrepresentation). Cell clone formation assay, clone appeared to be visible to the naked eye plate, and calculate the number of colonies formed after culture. By scratch assay and Transwell migration assay. The cell migration, cell migration distance measurement, recording of transmembrane cells number. Compared the results of three groups of different culture time was P, 0.05. experimental group, blank vector group and control group, the number of colonies formed respectively (414 + 29), (426 + 30), (381 + 59), compared to the three groups, P 0.05. experimental group, empty vector group, the control group cells relative migration distance were (6.71 + 0.10), (9.82 + 0.12), (9.98 + 0.10) mm, the experimental group cell migration distance less than the empty vector group and the control group (P < 0.05). The experimental group, empty vector group, the control group in mesangial cells respectively (37.33 + 1.53), (62.67 + 5.03), (62.13 + 4.36), experimental cells less than no-load group and the control group (P < 0.05). Conclusion over expression of CYFIP1 in nasopharyngeal carcinoma cell line CNE2 migration was inhibited by.CYFIP1 abnormal expression and nasopharyngeal carcinoma infiltration and development relevant.

【作者单位】：广西医科大学第一附属医院;
【基金】：广西壮族自治区自然科学基金资助项目(2014GXNSFAA118228)
【分类号】：R739.63

【相似文献】