p-Akt-mTOR-p70S6K信号通路蛋白与卵巢癌临床病理特征及化疗耐药的相关性

发布时间：2018-04-05 15:22

本文选题：卵巢癌　切入点：紫杉醇　出处：《中南大学学报(医学版)》2017年08期

【摘要】：目的:研究磷酸化蛋白激酶B(p-Akt)-雷帕霉素靶蛋白(mTOR)-p70核糖体蛋白S6激酶(p70S6K)信号通路中的p-Akt,mTOR和p70S6K 3种蛋白与卵巢癌临床病理特征及化学药物治疗(简称化疗)耐药的关系。方法:采用免疫组织化学方法检测18例卵巢癌化疗耐药和25例化疗敏感组织中p-Akt,mTOR和p70S6K蛋白的表达,分析这些蛋白与卵巢癌临床病理特征及化疗耐药的相关性。结果:p-Akt蛋白在卵巢浆液性癌、黏液性癌和子宫内膜样癌中的表达分别为77.14%,50.00%和66.67%,三者比较差异无统计学意义(P0.05);在高、中分化和低分化癌中的表达分别为73.33%和75.00%,两者比较差异无统计学意义(P0.05);在I~Ⅱ期和Ⅲ~Ⅳ期中的表达分别为18.18%和93.75%,差异有统计学意义(P0.05)。mTOR蛋白在卵巢浆液性癌、黏液性癌和子宫内膜样癌中的表达分别为77.14%,100.00%和83.33%,三者比较差异无统计学意义(P0.05);在高、中分化和低分化癌中的表达分别为80.00%和78.57%,两者比较差异无统计学意义(P0.05);在I~Ⅱ期和Ⅲ~Ⅳ期中的表达分别为27.27%和96.88%,差异有统计学意义(P0.05)。p70S6K蛋白在卵巢浆液性癌、黏液性癌和子宫内膜样癌中的表达分别为80.00%,100.00%和100.00%,三者比较差异无统计学意义(P0.05);在高、中分化和低分化癌中的表达分别为93.33%和78.57%,两者比较差异无统计学意义(P0.05);在I~Ⅱ期和Ⅲ~Ⅳ期中的表达分别为45.45%和96.88%,差异有统计学意义(P0.05)。p-Akt蛋白在卵巢癌化疗耐药和敏感组织中的表达率分别为88.89%及64.00%;mTOR蛋白在耐药和敏感组织中的表达率分别为94.44%及68.00%;p70S6K蛋白在耐药和敏感组织中的表达率分别为100.00%及72.00%,3种蛋白在耐药组中的表达均高于敏感组(P0.05)。结论:p-AktmTOR-p70S6K信号通路中的3种蛋白可能参与卵巢癌的侵袭和转移,这3种蛋白的表达上调可能与卵巢癌化疗耐药有关,在临床上可作为预测卵巢癌化疗耐药的标志。
[Abstract]:Aim: to study the relationship between p-AktnmTOR and p70S6K in the signal pathway of phosphorylated protein kinase (PK-B) -Aktna-rapamycin target protein mTOR-p70 ribosomal protein S6 kinase (p70S6K) and clinicopathological characteristics of ovarian cancer and chemotherapeutic resistance (chemotherapeutic).Methods: the expression of p-AktmTOR and p70S6K proteins in 18 cases of ovarian cancer and 25 cases of chemotherapy-sensitive tissues were detected by immunohistochemical method. The correlation between these proteins and clinicopathological features and chemotherapeutic resistance of ovarian cancer was analyzed.Results the expression of the protein in ovarian serous carcinoma, mucinous carcinoma and endometrial carcinoma were 77.14% and 66.67%, respectively.The expression rates in mucinous carcinoma and endometrial carcinoma were 100.00% and 83.33%, respectively. There was no significant difference among the three groups (P 0.05).In mucinous carcinoma and endometrial carcinoma, the expression rates were 100.00% and 100.00%, respectively. There was no significant difference among the three groups (P 0.05).Conclusion the expression of three proteins may be involved in the invasion and metastasis of ovarian cancer, and the up-regulation of these three proteins may be related to the chemoresistance of ovarian cancer, which may be used as a marker for predicting chemoresistance in ovarian cancer clinically.
【作者单位】：中南大学湘雅医院妇科;江西萍乡湘雅萍矿合作医院妇科;
【分类号】：R737.31

【相似文献】