关于人类结直肠癌的肠道微生物群落的生态统计分析

发布时间：2018-04-08 11:06

本文选题：微生物群落　切入点：结直肠癌　出处：《昆明理工大学》2017年硕士论文

【摘要】：结直肠癌(Colorectal Cancer,CRC)是世界上常见的恶性肿瘤之一。研究结果表明,肠道微生物群落组成的变化可能诱发结直肠癌的发生,因此可以作为这一疾病的敏感信号。然而,结直肠癌是否与肠道微生物群落的维持机制有关仍然知之甚少。本文从生态统计学角度对与结直肠癌有关组织的微生物群落的多样性及其分布进行统计分析,主要内容为:第一,对微生物群落建立线性模型研究其分布关系。进而利用扩展的Taylor幂法则研究结直肠癌患者与健康人相关组织的微生物群落的空间异质性(种间异质性及种内异质性),一方面对8名结直肠癌患者的癌变组织的异质性与邻近的正常组织的异质性进行比较,另一方面对46位结直肠癌患者患病组织与56位健康人相同部位的组织的异质性进行比较,以探讨罹患结直肠癌是否与组织的微生物群落的空间异质性的改变有关。第二,用Ewens和Etienne两种抽样方法对来自8位结直肠癌患者的癌变组织和邻近正常组织的采样数据以及46位结直肠癌患者和56位健康人的采样数据进行中性检验,运用卡方检验比较两种方法的差异,总结中性率。对中性模型里的基础多样性参数和Simpson多样性指数进行了详细阐述,深入探讨它们之间的关系。研究将回归、检验、估计等相关的统计方法应用并扩展到生态领域中的结直肠癌有关的肠道微生物群落中,试图在这一新的环境群落中探索相关组织间的生态机制和聚集、维护能力的差异,结果表明癌变组较正常对照组的种间异质性和多样性发生了相应的变化,初步结果对于展开后续关于肠道微生物疾病相关的生态统计研究是有意义的。
[Abstract]:Colorectal carcinoma (CRC) is one of the most common malignant tumors in the world.The results indicate that the change of intestinal microbial community may induce the occurrence of colorectal cancer, so it can be used as a sensitive signal of this disease.However, little is known about whether colorectal cancer is related to the maintenance mechanism of the intestinal microbial community.In this paper, the diversity and distribution of microbial communities in tissues related to colorectal cancer are analyzed statistically from the perspective of ecological statistics. The main contents are as follows: first, establish a linear model to study the distribution of microbial communities.Furthermore, the spatial heterogeneity (interspecific heterogeneity and intraspecific heterogeneity) of the microbial communities in the tissues related to colorectal cancer patients and healthy people were studied by using the extended Taylor power law. On the one hand, the cancer tissues of 8 patients with colorectal cancer were studied.The heterogeneity was compared with that of the adjacent normal tissue.On the other hand, the heterogeneity of the same site in 46 patients with colorectal cancer and 56 healthy people was compared to explore whether colorectal cancer is related to the spatial heterogeneity of microorganism community.Second, samples from cancerous tissues and adjacent normal tissues from 8 patients with colorectal cancer and those from 46 colorectal cancer patients and 56 healthy people were tested for neutral test using Ewens and Etienne sampling methods.The differences between the two methods were compared by chi-square test, and the neutral rate was summarized.The basic diversity parameters and Simpson diversity index in neutral model are discussed in detail, and the relationship between them is discussed.The study applied and extended regression, testing, estimation and other statistical methods to the intestinal microbial community associated with colorectal cancer in the ecological field, trying to explore the ecological mechanism and aggregation of related tissues in this new environmental community.The difference of maintenance ability indicated that the interspecific heterogeneity and diversity of cancer group were different from those of normal control group, and the preliminary results were significant for the subsequent ecological statistical study on intestinal microbial diseases.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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