胃癌组织中FOXC1与E-Cadherin基因的表达及与临床病理特征的关系

发布时间：2018-04-10 12:46

本文选题：FOXC1 + E-Cadherin　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:探讨叉头框蛋白C1(FOXC1)基因与E-钙粘附蛋白(E-Cadherin)基因在胃癌组织、癌旁组织中的表达情况,两者之间是否具有统计学差异;并就其表达与胃癌病人临床病理特征的关系进行分析,为胃癌患者的临床诊断、治疗、预后提供可能的理论依据。方法:选取2014年3月至2016年6月青岛大学附属医院急诊普外科住院的胃癌手术病人102例(均以病理检查明确诊断),剔除不符合纳入标准者3例(术前行其他治疗者2例;同时合并有其他脏器肿瘤者1例),剩余99例。分别取胃癌病人手术切除后的癌旁组织(切除距肿瘤边缘6cm)及胃癌组织制作的病理石蜡切片(以上胃癌组织及癌旁组织的选取均经病理科老师在显微镜下协助证实),选取的胃癌组织标本作为胃癌组,癌旁组织作为癌旁组。使用免疫组织化学(非生物素二步法)检测胃癌组及癌旁组中FOXC1基因与E-Cadherin基因的表达情况,然后分析其在统计学上有无差异性。然后根据胃癌病人的年龄、性别、有无淋巴结转移、浸润深度、TNM分期、肿瘤分化程度等临床指标进行分组比较,探讨FOXC1基因及E-Cadherin基因的表达与胃癌病人临床病理特征之间的关系。结果:(1)FOXC1基因在胃癌组织、癌旁组织中的阳性表达率分别为73.74%、44.44%,FOXC1基因在胃癌组织、癌旁组织中的表达存在明显统计学差异(P0.05)。(2)E-Cadherin基因在胃癌组织及癌旁组织中的阳性表达率分别为29.29%、73.74%,E-Cadherin基因在胃癌组织和癌旁组织中的表达存在明显的统计学差异(P0.05)。(3)FOXC1基因的表达与胃癌病人的性别、年龄无统计学差异(P0.05),FOXC1基因的表达与肿瘤的组织分型、肿瘤TNM分期、组织浸润深度、有无淋巴结转移存在明显统计学差异(P0.05);E-Cadherin基因的表达与胃癌病人的年龄、性别无明显的统计学差异(P0.05),与肿瘤的组织分型、肿瘤的TNM分期、有无淋巴结转移有统计学差异(P0.05);FOXC1基因与E-Cadherin基因在胃癌组织中的表达存在负相关性,X2值为54.31,r=-0.887,(P0.05)。FOXC1基因与E-Cadherin基因在癌旁组织中的表达存在正相关性,X2值为69.02,r=0.789,(P0.05)。结论:FOXC1及E-Cadherin基因可能参与胃癌的发生、发展过程,可能作为诊断胃癌病人临床及预后的一项指标,或可能成为胃癌靶向治疗的一个新靶点。
[Abstract]:Objective: to investigate the expression of forkhead protein C1FOXC1 gene and E-cadherin gene in gastric cancer tissues and adjacent tissues, and whether there is statistical difference between them.The relationship between its expression and clinicopathological features of gastric cancer patients was analyzed to provide possible theoretical basis for clinical diagnosis, treatment and prognosis of gastric cancer patients.Methods: 102 patients with gastric cancer were selected from March 2014 to June 2016 in emergency department of general surgery, Qingdao University affiliated Hospital (all patients were diagnosed by pathological examination, 3 cases were excluded from the inclusion criteria) (2 cases received other treatment before operation);At the same time, other organ tumors were found in 1 case and the remaining 99 cases.The paracancerous tissues (6 cm from the margin of the tumor) and the pathological paraffin sections made by the gastric cancer tissues were taken from the patients with gastric cancer after surgical resection. (the selection of the above gastric cancer tissues and the adjacent tissues were all assisted by the pathologist under the microscope.The specimens of gastric cancer were selected as gastric cancer group,Paracancerous tissue is used as the paracancerous group.The expression of FOXC1 gene and E-Cadherin gene in gastric cancer group and paracancerous group were detected by immunohistochemistry (non-biotin two-step method), and then the statistical differences were analyzed.Then according to the age, sex, lymph node metastasis, TNM stage and tumor differentiation of gastric cancer patients, the patients were grouped and compared.To investigate the relationship between the expression of FOXC1 gene and E-Cadherin gene and the clinicopathological features of gastric cancer patients.Results the positive expression rates of FOXC1 gene in gastric cancer tissues and adjacent tissues were 73.74 and 44.44, respectively.And the sex of patients with gastric cancer,There was no significant difference in age between the expression of FOXC1 gene and tumor type, TNM stage, depth of infiltration, lymph node metastasis and age of patients with gastric cancer.There was no significant statistical difference in sex (P 0.05), and there was no significant difference between sex and tumor tissue type, tumor TNM stage,Conclusion the gene of: FOXC1 and E-Cadherin may be involved in the occurrence and development of gastric cancer, and may be used as a marker for clinical diagnosis and prognosis of gastric cancer patients, or as a new target for the targeted therapy of gastric cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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