二甲双胍在喉癌Hep-2细胞中对5-氟尿嘧啶、顺铂、紫杉醇的作用及机制探讨

发布时间：2018-04-11 05:26

本文选题：二甲双胍 + 喉肿瘤　；参考：《临床耳鼻咽喉头颈外科杂志》2017年07期

【摘要】：目的:检测5-氟尿嘧啶联合二甲双胍对喉癌Hep-2细胞增殖抑制作用及化疗敏感影响。探究二甲双胍联合顺铂、紫杉醇、5-氟尿嘧啶作用于Hep-2细胞AMPK通路信号分子的表达变化。方法:体外培养Hep-2细胞,二甲双胍联合5-氟尿嘧啶作用细胞72h,MTT检测增殖情况。二甲双胍联合顺铂、5-氟尿嘧啶、紫杉醇作用细胞72h,Western blot检测P21、Cyclin D1、AMPK-α表达。结果:5-氟尿嘧啶以及二甲双胍可抑制Hep-2细胞增殖。二甲双胍联合低浓度5-氟尿嘧啶,细胞生长抑制率升高;二甲双胍联合高浓度5-氟尿嘧啶生长抑制率无明显差异,两药在Hep-2细胞中相互拮抗。Western blot显示,顺铂、5-氟尿嘧啶增加细胞中上述蛋白表达,紫杉醇减少细胞中Cyclin D1表达。对比单独用药组,联用二甲双胍均引起Hep-2细胞中上述蛋白表达变化。结论:5-氟尿嘧啶抑制Hep-2细胞增殖,二甲双胍拮抗5-氟尿嘧啶对Hep-2细胞抗肿瘤效应,其拮抗作用及对顺铂、紫杉醇的影响部分通过AMPK、P21及Cyclin D1相关通路发生作用且与周期阻滞存在密切关系。
[Abstract]:Aim: to detect the inhibitory effect of 5 fluorouracil combined with metformin on the proliferation and chemosensitivity of laryngeal carcinoma Hep-2 cells.To investigate the effect of metformin combined with cisplatin and paclitaxel 5-fluorouracil on the expression of AMPK signaling molecules in Hep-2 cells.Methods: Hep-2 cells were cultured in vitro and cultured with metformin and 5-fluorouracil.Metformin combined with cisplatin 5-fluorouracil and paclitaxel treated cells for 72 h were used to detect the expression of AMPK- 伪 by Western blot.Results:% 5 fluorouracil and metformin could inhibit the proliferation of Hep-2 cells.The cell growth inhibition rate of metformin combined with low concentration 5-fluorouracil was increased, but there was no significant difference between metformin and high concentration 5-fluorouracil. Western blot showed that the two drugs antagonized each other in Hep-2 cells.Cisplatin 5-fluorouracil increased the expression of above-mentioned protein, while paclitaxel reduced the expression of Cyclin D1.Both metformin and metformin induced changes in the expression of these proteins in Hep-2 cells.Conclusion the proliferation of Hep-2 cells was inhibited by 5 fluorouracil, and metformin antagonized the antitumor effect of 5 fluorouracil on Hep-2 cells, and the antagonism of 5 fluorouracil and cisplatin on Hep-2 cells.The effect of paclitaxel was partly mediated by AMPK P21 and Cyclin D1 pathways and was closely related to cycle arrest.
【作者单位】：中国人民解放军第401医院耳鼻咽喉科;中国人民解放军白求恩国际和平医院耳鼻咽喉头颈外科;
【分类号】：R739.65

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