RNAscope技术与IHC在判断乳腺癌组织ERα表达的比较研究

发布时间：2018-04-11 09:48

  本文选题：RNAscope + 免疫组化　； 参考：《重庆医科大学》2017年硕士论文

【摘要】：目的:雌激素受体α(Estrogen receptorα,ERα)在乳腺癌的发生发展中起重要作用,临床上用于判断乳腺癌组织ERα表达的主要方法是免疫组化(IHC),但是IHC结果受诸多因素的影响。本文应用RNAscope技术来检测乳腺癌组织ERα的表达,并将RNAscope的结果与IHC的结果进行对比分析,旨在进一步探讨RNAscope技术的临床应用价值。方法:收集人乳腺癌组织标本(72例,47例ERα阳性以及25例ERα阴性),将收集到的标本分成三份,一份用来提取RNA,进行RT-q PCR,一份用来进行RNAscope原位杂交和IHC,一份备用;然后构建标准曲线,进行绝对定量RT-q PCR,并将绝对定量RT-q PCR以及RNAscope的结果与IHC进行对比分析。结果:ERα阳性乳腺癌组织标本的RT-qPCR结果和RNAscope结果显著高于ERα阴性乳腺癌组织标本,RT-q PCR的结果与IHC结果的一致性并不明显,RNAscope的结果与IHC结果的一致性较好,在对不一致的组织标本分析中我们发现有3例IHC判断为ERα阴性而RNAscope判断为阳性的标本最后应该诊断为阳性,有2例IHC判断为ERα阳性RNAscope判断为阴性的标本最后应该诊断为阴性,有1例IHC判断为ERα阴性RNAscope判断为阳性的标本最后应该诊断为阴性。结论:1.RNAscope结果与IHC结果具有较好的一致性,RT-q PCR结果与IHC结果一致性不明显;且RNAscope操作较RT-q PCR操作简便,还具有可视化,与RT-q PCR相比,具有明显优势。2.为了提高ERα的诊断,RNAscope技术可以作为IHC的一种补充实验技术。
[Abstract]:Objective: estrogen receptor 伪 strogen receptor 伪 er 伪 plays an important role in the occurrence and development of breast cancer. The main method for judging the expression of ER 伪 in breast cancer is immunohistochemistry, but the results of IHC are affected by many factors.In this paper, the expression of ER 伪 in breast cancer tissue was detected by RNAscope technique, and the results of RNAscope and IHC were compared and analyzed in order to further explore the clinical application value of RNAscope technique.Methods: a total of 72 human breast cancer specimens were collected from 47 cases of ER 伪 positive and 25 cases of ER 伪 negative. The collected specimens were divided into three parts, one for RT-q, one for RNAscope in situ hybridization and one for RNAscope.Then the standard curve was constructed, and the absolute quantitative RT-q PCR and RNAscope results were compared with IHC.Results the results of RT-qPCR and RNAscope were significantly higher in the breast cancer tissue samples with ER 伪 positive than those in the samples with ER 伪 negative breast cancer. The results of RT-q PCR and IHC were not in good agreement with the results of IHC.In the analysis of inconsistent tissue specimens, we found that three specimens with ER 伪 negative for IHC and positive for RNAscope should be diagnosed as positive in the end, and 2 samples with ER 伪 positive RNAscope negative in IHC should be diagnosed as negative in the end.In one case, ER 伪 negative RNAscope positive specimen diagnosed by IHC should be diagnosed as negative in the end.Conclusion: 1. The results of RNAscope and IHC have good consistency. The results of RT-q PCR and IHC are not consistent, and the operation of RNAscope is simpler and more visual than that of RT-q PCR. Compared with RT-q PCR, it has obvious advantage. 2.In order to improve the diagnostic ability of ER 伪, it can be used as a supplementary experimental technique for IHC.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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