乳腺癌患者外周血循环肿瘤细胞阴性富集法检测及其临床意义

发布时间：2018-04-12 06:29

  本文选题：循环肿瘤细胞 + 乳腺癌　； 参考：《中华肿瘤防治杂志》2017年05期

【摘要】：目的循环肿瘤细胞(circulating tumor cells,CTC)的检测作为一种新兴的检测方法,在临床实践中拥有广阔的应用前景。本研究通过阴性富集法检测乳腺癌患者外周血CTC,探讨CTC水平与其分子分型及临床特征的相关性,研究其临床意义。方法选取连云港市第一人民医院2015-08-18-2016-02-20接受CTC检测的乳腺癌患者152例为研究对象,收集临床资料,选择同期该院29例乳腺良性疾病患者作为对照组。采用免疫磁珠阴性富集及免疫荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测CD45~-/CEP~+/DAPI~+的CTC,并结合乳腺癌分子分型和临床特征进行统计学分析。结果 152例乳腺癌患者与29例乳腺良性疾病患者进行ROC曲线分析,比较不同切点的敏感度及特异度,经计算Youden指数最高为0.390,对应切点为≥2.5,故取CTC个数≥3为阳性指标。152例乳腺癌患者中CTC阳性率Ⅰ~Ⅱ期为41.5%,Ⅲ期为60.0%,Ⅳ期为73.9%,差异有统计学意义,χ~2=9.360,P=0.009。CTC与乳腺癌分子分型无相关性,P0.05,与肿瘤原发灶分期、区域淋巴结转移、激素受体状况及HER2表达均无相关性,P0.05。但与是否发生远处转移密切相关,χ~2=5.863,P=0.015。结论 CTC与乳腺癌分子分型无明显相关,但与患者临床分期、是否发生转移密切相关,检测CTC有利于更准确的评估病情。
[Abstract]:Objective as a new method, the detection of circulating tumor cells has a broad application prospect in clinical practice.In this study, the negative enrichment method was used to detect the level of CTC in peripheral blood of breast cancer patients, and to explore the correlation between the level of CTC and its molecular typing and clinical features.Methods A total of 152 breast cancer patients with CTC were selected from the first people's Hospital of Lianyungang City, 2015-08-2016-02-20. The clinical data were collected and 29 patients with benign breast diseases were selected as the control group.Immunofluorescence in situ hybridization in situ hybridization (fish) was used to detect the CTCs of CD45% CEP- / DAPI-, and the molecular typing and clinical characteristics of breast cancer were analyzed by using immunomagnetic bead negative enrichment and immunofluorescence in situ hybridization (fish) technique.Results the sensitivity and specificity of different cut points were compared by ROC curve analysis in 152 patients with breast cancer and 29 patients with benign breast diseases.The highest Youden index is 0.390, and the corresponding point is 鈮，

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