培美曲塞脂质体的制备及其对乳腺癌的作用观察

发布时间：2018-04-13 07:21

本文选题：培美曲塞 + 脂质体　；参考：《上海交通大学学报(医学版)》2017年02期

【摘要】：目的·制备培美曲塞脂质体,观察其对乳腺癌细胞MCF-7及荷瘤裸鼠模型肿瘤的抑制作用。方法·采用薄膜分散法制作脂质体,CCK-8法检测其对MCF-7细胞的抑制作用;建立Balb/c裸鼠荷瘤模型,通过尾静脉给药以检测其抑瘤作用。结果·培美曲塞脂质体可抑制MCF-7细胞增殖,当培美曲塞浓度分别为0.20、0.40和10.00μg/m L时,实验组(培美曲塞脂质体)的细胞生存率显著小于对照组(等浓度梯度培美曲塞),P值分别为0.013、0.035和0.041。与空白组(等剂量PBS)比较,实验组(培美曲塞脂质体)和对照组(等剂量培美曲塞)Balb/c裸鼠肿瘤的体积和质量均显著减小;实验组肿瘤的体积和质量也均显著小于对照组(均P=0.000)。结论·相对于培美曲塞原料药物,脂质体能抑制MCF-7细胞的增殖,通过尾静脉给药能有效抑制肿瘤在Balb/c裸鼠体内的生长。
[Abstract]:Aim to prepare pemetrexide liposome and observe its inhibitory effect on breast cancer cell line MCF-7 and tumor-bearing nude mice.Methods the inhibitory effect of liposome CCK-8 on MCF-7 cells was detected by membrane dispersion method, and the tumor-bearing model of Balb/c nude mice was established.Results the proliferation of MCF-7 cells was inhibited by pemetrexide liposome at concentrations of 0.20 渭 g / mL and 10.00 渭 g / mL, respectively.The cell survival rate of the experimental group (pemetrexed liposome) was significantly lower than that of the control group (equal concentration gradient pemetrezepine P values were 0.013 ~ 0.035 and 0.041 respectively).Compared with the blank group (equal dose PBSs), the volume and mass of the tumor in the experimental group (pemetrexed liposome) and the control group (equal dose pemetrexide Balb / c) were significantly decreased, and the tumor volume and mass in the experimental group were significantly lower than those in the control group (all P0. 000).Conclusion compared with pemetrexide, liposome can inhibit the proliferation of MCF-7 cells and inhibit the growth of tumor in Balb/c nude mice by tail vein administration.
【作者单位】：上海交通大学医学院附属新华医院普外科;上海交通大学医学院附属新华医院药剂科;复旦大学附属妇产科医院乳腺外科;
【基金】：上海市卫生和计划生育委员会基金(20154Y0077) 上海高校选拔培养优秀青年教师科研专项基金(zzjdyx13087) 上海交通大学医学院附属新华医院基金(14YJ25)~~
【分类号】：R737.9

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