喉癌与HPV、EBV感染的相关性研究

发布时间：2018-04-14 05:12

本文选题：喉癌 + 声带息肉　；参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的探讨人乳头状瘤病毒(Human Papillomavirus,HPV)、EB病毒(Epstein-Barr virus,EBV)在喉癌发病过程中的相关性。方法取101例临床与病理确诊的喉癌石蜡包埋组织标本采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)和实时荧光定量PCR技术进行37种HPV亚型检测和EBV定量检测,并以43例临床与病理确诊的声带息肉石蜡包埋组织为对照,进行对比研究。结果喉癌组中HPV总阳性率为13.86%(14/101),声带息肉组中HPV总阳性率为9.3%(4/43)。HPV在喉癌和声带息肉组织中的阳性表达差异无统计学意义(P0.05)。喉癌组中EBV阳性率为63.37%(64/101),声带息肉组中EBV阳性率为13.95%(6/43)。EBV在喉癌及声带息肉组织中的阳性表达差异具有统计学意义(P0.05);通过不同分化程度的喉癌组织中EBV阳性率的相互比较,EBV在高分化型与中分化型喉癌组织中的阳性表达差异无统计学意义(P0.05),EBV在高分化型与低分化型、中分化型与低分化型喉癌组织中的阳性表达差异具有统计学意义(P0.05);在不同性别、年龄、病程以及民族的喉癌患者中EBV阳性率均无统计学意义。结论喉癌的发生发展与EBV感染密切相关,与HPV感染可能无相关性。本研究通过探讨喉癌的发生、发展与HPV、EBV感染之间的相关性,以及喉癌的性别、年龄、病程、民族、分化程度等相关因素与EBV感染的关系,为未来研究喉癌的病毒学致病机制以及临床上预防和治疗喉癌提供一定的依据。
[Abstract]:Objective to investigate the correlation between Epstein-Barr virus (EBV) and Epstein-Barr virus (EBV) in laryngeal carcinoma.Methods A total of 101 paraffin-embedded tissue specimens of laryngeal carcinoma were collected for 37 HPV subtypes and EBV quantitative detection by using DNA molecular fast flow hybridization gene chip typing technique and real-time fluorescence quantitative PCR technique.A comparative study was carried out on 43 cases of paraffin embedded tissues of vocal cord polyps diagnosed clinically and pathologically.Results the total positive rate of HPV was 13.86% in laryngeal carcinoma group and 14% / 101% in vocal cord polyp group. The total positive rate of 9.3%(4/43).HPV in vocal cord polyp group was not significantly different from that in laryngeal carcinoma and vocal cord polyp tissue (P 0.05).The positive rate of EBV in laryngeal carcinoma group was 63.37% and that in vocal cord polyp group was 63.37%. The positive rate of 13.95%(6/43).EBV in laryngeal carcinoma and vocal cord polyp was significantly higher than that in vocal cord polyp tissue (P 0.05). The ratio of EBV positive rate in laryngeal carcinoma tissues with different differentiation degree was different from that in vocal cord polyp group.There was no significant difference in the positive expression of EBV between well-differentiated and moderately differentiated laryngeal carcinomas. There was no significant difference in the expression of EBV between well-differentiated and poorly differentiated laryngeal carcinomas, and there was no significant difference in the expression of EBV between well-differentiated and poorly differentiated laryngeal carcinomas.There was significant difference in the positive expression of EBV between moderately differentiated and poorly differentiated laryngeal carcinoma tissues, but there was no significant difference in the positive rate of EBV among different sex, age, course of disease and ethnic group of laryngeal carcinoma.Conclusion the occurrence and development of laryngeal carcinoma are closely related to EBV infection, but not to HPV infection.In this study, we investigated the relationship between EBV infection and the occurrence, development of laryngeal carcinoma, sex, age, course of disease, nationality and differentiation of laryngeal carcinoma.It provides a basis for the study of virological mechanism and clinical prevention and treatment of laryngeal carcinoma.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.65

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