核糖体蛋白MPS-1在多柔比星诱导胃癌细胞凋亡中的作用

发布时间：2018-04-16 16:04

本文选题：胃肿瘤 + 多柔比星　；参考：《肿瘤》2017年09期

【摘要】：目的:探讨核糖体蛋白MPS-1(metallopanstimulin-1)在多柔比星诱导胃癌AGS细胞凋亡中的作用。方法:分别应用实时荧光定量PCR和蛋白质印迹法检测MPS-1稳定下调的p SIREN-sh MPS-1-1和pSIREN-shMPS-1-2胃癌AGS细胞中MPS-1 m RNA和蛋白的表达。0、1和2μmol/L多柔比星处理p SIRENsh MPS-1-1和pSIREN-shMPS-1-2胃癌AGS细胞后,应用TUNEL法检测各组细胞的凋亡情况。构建MPS-1过表达重组载体p CMV4-FlagMPS-1,并将其转染至pSIREN-shMPS-1-2胃癌AGS细胞中(p SIRENsh MPS-1-2/MPS-1),再用0、1和2μmol/L多柔比星处理后,应用TUNEL法检测细胞的凋亡情况。应用蛋白质印迹法检测pSIRENsh MPS-1-1和pSIREN-shMPS-1-2胃癌AGS细胞及多柔比星处理的pSIREN-shMPS-1-2胃癌AGS细胞中剪切型多聚二磷酸腺苷核糖聚合酶[cleaved poly(ADP-ribose)polymerase,cleaved PARP]的表达。结果:pSIREN-shMPS-1-2胃癌AGS细胞中MPS-1 m RNA和蛋白的表达水平均明显下降(P值均0.01)。0、1和2μmol/L多柔比星处理后,pSIREN-shMPS-1-2胃癌AGS细胞的凋亡率明显上升(P值均0.05),pSIREN-shMPS-1-2/MPS-1胃癌AGS细胞的凋亡率则下降(P值均0.05)。pSIREN-shMPS-1-2胃癌AGS细胞及0、1和2μmol/L多柔比星处理后的pSIREN-shMPS-1-2胃癌AGS细胞中cleaved PARP表达水平均上调(P值均0.05)。结论 :MPS-1下调在多柔比星诱导的胃癌AGS细胞凋亡中起促进作用。
[Abstract]:Aim: to investigate the role of ribosomal protein MPS-1 metallopanstimulin-1 in apoptosis of gastric cancer AGS cells induced by doxorubicin.Methods: the expression of MPS-1 m RNA and protein in p SIREN-sh MPS-1-1 and pSIREN-shMPS-1-2 AGS cells, which were stably down-regulated by MPS-1, were detected by real-time fluorescence quantitative PCR and Western blotting, respectively. The expression of MPS-1 m RNA and protein in P SIRENsh MPS-1-1 and pSIREN-shMPS-1-2 AGS cells were treated with doxorubicin and 2 渭 mol/L respectively.Apoptosis was detected by TUNEL assay.MPS-1 overexpression vector pCMV4-FlagMPS-1 was constructed and transfected into pSIREN-shMPS-1-2 gastric cancer AGS cells. After treated with 0 渭 mol/L doxorubicin and 0 渭 mol/L doxorubicin, the apoptosis of the cells was detected by TUNEL assay.The expression of cleaved polyadenosine diphosphate polymerase (cleaved) in pSIRENsh MPS-1-1 and pSIREN-shMPS-1-2 gastric cancer AGS cells and pSIREN-shMPS-1-2 AGS cells treated with doxorubicin was detected by Western blot.Results the expression level of MPS-1 m RNA and protein in AGS cells of pSIREN-shMPS-1-2 decreased significantly (P < 0.05). The apoptotic rate of AGS cells was significantly increased after treatment with doxorubicin (0.01 渭 mol/L) and doxorubicin (2 渭 mol/L). The apoptotic rate of AGS cells was decreased by 0.05pSIREN-shMPS-1-2pMPS-1 in gastric cancer cells treated with pSIREN-shMPS-1-2 and 2 渭 mol/L doxorubicin respectively.The expression of cleaved PARP in pSIREN-shMPS-1-2 gastric cancer AGS cells treated with 0 渭 mol/L doxorubicin and 0 1 渭 mol/L doxorubicin were all up-regulated in 0.05).pSIREN-shMPS-1-2 gastric cancer AGS cells and 0. 05 渭 mol/L doxorubicin treatment.Conclusion the down-regulation of 10% MPS-1 may promote the apoptosis of gastric cancer AGS cells induced by doxorubicin.
【作者单位】：复旦大学附属中山医院肝肿瘤内科;
【分类号】：R735.2

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