多发性骨髓瘤患者血清miR-193b表达变化及临床意义

发布时间：2018-04-16 21:26

本文选题：多发性骨髓瘤 + 微小RNA-b　；参考：《山东医药》2017年48期

【摘要】：目的探讨多发性骨髓瘤(MM)患者血清miR-193b表达变化及临床意义。方法选取MM患者42例(MM组),其中Ig G型16例、Ig A型9例、其他17例,体检健康者35例(对照组)。采用实时荧光定量PCR(RT-q PCR)法检测血清miR-193b相对表达量;采用Spearman相关分析法分析MM患者血清miR-193b表达与β2微球蛋白(β2M)、轻链κ、轻链λ和乳酸脱氢酶(LDH)水平的关系;采用受试者工作特征(ROC)曲线评估各指标单独及联合检测对MM的诊断效能。结果 MM组和对照组血清miR-193b相对表达量分别为6.904±2.782、4.454±2.553,组间比较P0.01。Ig G型MM患者血清miR-193b相对表达量为8.001±2.479,Ig A型为5.268±1.849,其他为6.396±3.132;Ig G型高于Ig A型(P0.01),其余各型比较差异均无统计学意义(P均0.05)。MM患者血清miR-193b表达与血清β2M、轻链κ、轻链λ和LDH水平均无相关性(P均0.05)。血清miR-193b诊断MM的敏感性、准确性均高于轻链κ、轻链λ、β2M和LDH,血清miR-193b联合轻链κ、轻链λ、β2M或LDH诊断MM的敏感性、准确性均高于各单项指标。结论 MM患者血清miR-193b表达上调;检测血清miR-193b表达变化可用于MM的辅助诊断及临床分型。
[Abstract]:Objective to investigate the changes and clinical significance of serum miR-193b expression in patients with multiple myeloma.Methods 42 patients with MM were enrolled in this study, including 16 patients with IgG type Ig A, 9 with IgA, 17 with others, and 35 healthy subjects (control group).The relative expression of serum miR-193b was detected by real-time fluorescence quantitative PCR(RT-q assay, and the relationship between the expression of serum miR-193b and 尾 2 microglobulin (尾 2 microglobulin, light chain 魏, light chain 位 and lactate dehydrogenase PCR(RT-q) in MM patients was analyzed by Spearman correlation analysis.Objective: to evaluate the diagnostic effectiveness of individual and joint detection of MM by using the ROCcurve of the subjects.Results the relative expression of serum miR-193b in MM group and control group was 6.904 卤2.782 卤4.454 卤2.553, respectively. The relative expression of serum miR-193b in MM patients with P0.01.Ig G type was 8.001 卤2.479 (5.268 卤1.849), and the other 6.396 卤3.132 Ig G type was higher than that of IgA group (P 0.01). There was no significant difference between the other groups.There was no correlation between serum miR-193b expression and serum 尾 2M, light chain 魏, light chain 位 and LDH levels in patients with 0.05).MM.The sensitivity and accuracy of serum miR-193b in the diagnosis of MM were higher than those of light chain 魏, light chain 位, 尾 2m and LDH, serum miR-193b combined with light chain 魏, light chain 位, 尾 2m or LDH.Conclusion the expression of serum miR-193b is up-regulated in patients with MM, and the change of serum miR-193b expression can be used for the auxiliary diagnosis and clinical classification of MM.
【作者单位】：南通大学附属医院;
【基金】：国家自然科学基金面上项目(81672099) 江苏省重点研发专项(BE2015654) 江苏省“科教强卫”工程青年医学人才项目(QNRC2016695) 南通市民生科技创新和示范推广计划项目(MS120170008-6)
【分类号】：R733.3

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