巨噬细胞炎症蛋白-1β对人舌鳞癌细胞CAL-27增殖和凋亡的影响

发布时间：2018-04-17 08:19

本文选题：舌癌 + 趋化因子　；参考：《南方医科大学学报》2017年08期

【摘要】：目的探讨β亚族趋化因子受体5(CCR5)在不同人舌鳞癌细胞株的表达情况以及其配体巨噬细胞炎症蛋白-1β(MIP-1β)对人舌鳞癌细胞株CAL-27增殖和凋亡的影响。方法采用蛋白印迹法和免疫荧光染色对3种人舌鳞癌细胞株(CAL-27、UM-1、Tca-8113)MIP-1β的相应受体CCR5的表达进行检测;根据MIP-1β刺激浓度的不同,细胞被随机分为4组:0 ng/m L组(对照组)、10 ng/m L组、20 ng/m L组、40 ng/m L组。用CCK-8试剂盒(Cell Counting Kit-8)检测MIP-1β在刺激12、24、48 h时,对CAL-27细胞增殖的影响;实验分组同前,分别用0、10、20、40 ng/m L IP-10处理24 h后收集细胞,用Annexin V/PI双染流式细胞仪检测CAL-27细胞的凋亡情况。结果 CCR5在3种人舌鳞癌细胞株均有表达,并且CCR5在3株细胞的胞膜及胞质内均有染色;和对照组相比,MIP-1β对CAL-27细胞有促进增殖的作用。在12 h、24 h时,3种浓度的MIP-1β均能够促进CAL-27细胞增殖(P0.05);48 h时,10 ng/m L、20 ng/m L的MIP-1β均能够促进CAL-27细胞增殖(P0.05),但40 ng/m L的MIP-1β对CAL-27细胞的增殖无影响(P0.05);在24 h时,40 ng/m L MIP-1β组的细胞凋亡率显著高于对照组(P0.05),而10 ng/m L、20 ng/m L MIP-1β组细胞凋亡率和对照组之间均无显著差异(P0.05)。结论 CCR5在3种人舌鳞癌细胞株的胞膜及胞质内均有表达;MIP-1β对CAL-27细胞有促进增殖的作用,但相对较高浓度的MIP-1β对CAL-27细胞的凋亡也有促进作用。随着较高浓度MIP-1β作用时间的延长,促增殖作用减弱,而这种减弱可能是由MIP-1β的促凋亡作用的逐步发挥所引起的。
[Abstract]:Objective to investigate the expression of 尾 subfamily chemokine receptor 5 (CCR5) in different human tongue squamous cell carcinoma cell lines and the effect of its ligands, macrophage inflammatory protein 1 尾 (MIP-1 尾) on the proliferation and apoptosis of human tongue squamous cell carcinoma cell line CAL-27.Methods the expression of MIP-1 尾 receptor CCR5 in three human tongue squamous cell carcinoma cell lines CAL-27UM-1, Tca-8113 and MIP-1 尾 was detected by Western blotting and immunofluorescence staining.The cells were randomly divided into 4 groups: 0 ng/m / L (control group, 10 ng/m / L) and 20 ng/m / L group (40 ng/m / L).CCK-8 Counting Kit-8 was used to detect the effect of MIP-1 尾 on the proliferation of CAL-27 cells after 48 h stimulation, and the cells were collected after 24 h treatment with the same ng/m L IP-10 and Annexin V/PI double staining flow cytometry was used to detect the apoptosis of CAL-27 cells.Results CCR5 was expressed in three human tongue squamous cell carcinoma cell lines, and CCR5 was stained in the cell membrane and cytoplasm of the three cell lines, and MIP-1 尾 could promote the proliferation of CAL-27 cells compared with the control group.Three concentrations of MIP-1 尾 could promote the proliferation of CAL-27 cells at 12 h and 24 h. MIP-1 尾 of 10 ng/m L and 20 ng/m L could promote the proliferation of CAL-27 cells at 48 h. However, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells, and at 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells. At 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells, and at 24 h, 40 ng/m L MIP-1 尾 had no effect on the proliferation of CAL-27 cells.The rate of apoptosis was significantly higher than that of the control group (P 0.05), but there was no significant difference between the 10 ng/m L ~ + 20 ng/m L MIP-1 尾 group and the control group (P 0.05).Conclusion the expression of CCR5 in the membrane and cytoplasm of three human tongue squamous cell carcinoma cell lines can promote the proliferation of CAL-27 cells, but the relatively high concentration of MIP-1 尾 can also promote the apoptosis of CAL-27 cells.With the prolongation of high concentration of MIP-1 尾, the proliferative effect was weakened, which may be caused by the gradual exertion of the apoptotic effect of MIP-1 尾.
【作者单位】：南方医科大学口腔医院//广东省口腔医院口腔颌面外科;南方医科大学口腔医院//广东省口腔医院牙体牙髓科;
【基金】：国家自然科学基金(81670950) 广州市科技计划项目(2014Y2-00109) 广东省科技计划项目(2014A020212549)~~
【分类号】：R739.86

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